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学科主题临床医学
Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice
Xu, Feng1; Ye, Ying-Jiang1; Liu, Wei1; Kong, Meng1; He, Yu2; Wang, Shan1
刊名SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
2010-06-01
DOI10.3109/00365521003650180
45期:6页:707-713
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CYTOTOXIC T-LYMPHOCYTES ; INDUCE ANTITUMOR IMMUNITY ; CARCINOMA-CELLS ; TUMOR-GROWTH ; IN-VIVO ; FUSIONS ; IMMUNIZATION ; VACCINATION ; MODEL ; IMMUNOTHERAPY
英文摘要

Objective. Colorectal cancer (CRC) is one of the most common malignancies in the western world. More than 60% among patients will develop liver metastases. Although surgical resection is the first choice worldwide, at this point an effective approach for the treatment of patients with liver metastasis and cancer recurrence postoperation has not yet been found. The aim of this study is to investigate the role of the allogeneic dendritomas from fusion of DCs and metastatic colon cancer cells in the activation of anti-tumor immunity against colorectal cancer liver metastases. Material and methods. Hybrids were generated by fused allogeneic human peripheral blood dendritic cells with metastatic colon cancer SW620 cells using 50% polyethylene glycol (PEG). Induction of immune responses was assessed by ex vivo ELISPOT assays. A murine model of CRC liver metastasis was used by intrasplenic injection. The validity of the vaccine was observed by Vaccination CRC liver metastasis murine model with DC/tumor hybrids. Results. The hybrids highly express the major molecules of DCs and tumor cells. The number of hybrids pulsed CTL secreting IFN-gamma was significantly higher when compared to the DC controls (p < 0.01). In a therapeutic setting mice vaccinated with in vitro cultured hybrids produced strong cellular immune responses and significant inhibition of tumor growth, compared to sham vaccinated controls. Conclusions. Vaccination with hybrids can induces strong cellular responses and significant protection from challenge in SCID mouse metastatic CRC model.

语种英语
WOS记录号WOS:000277365200009
项目编号r0204003040531
资助机构Beijing Municipal Science &amp ; Technology Commission
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58530
专题北京大学第二临床医学院_胃肠外科
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Peoples Hosp, Surg Oncol Lab, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Peking Univ, Hosp 3, Dept Intens Care Unit, Beijing 100044, Peoples R China
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GB/T 7714
Xu, Feng,Ye, Ying-Jiang,Liu, Wei,et al. Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice[J]. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY,2010,45(6):707-713.
APA Xu, Feng,Ye, Ying-Jiang,Liu, Wei,Kong, Meng,He, Yu,&Wang, Shan.(2010).Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice.SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY,45(6),707-713.
MLA Xu, Feng,et al."Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice".SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 45.6(2010):707-713.
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