北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科  > 期刊论文
学科主题: 临床医学
题名:
Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice
作者: Xu, Feng1; Ye, Ying-Jiang1; Liu, Wei1; Kong, Meng1; He, Yu2; Wang, Shan1
刊名: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
发表日期: 2010-06-01
DOI: 10.3109/00365521003650180
卷: 45, 期:6, 页:707-713
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: CYTOTOXIC T-LYMPHOCYTES ; INDUCE ANTITUMOR IMMUNITY ; CARCINOMA-CELLS ; TUMOR-GROWTH ; IN-VIVO ; FUSIONS ; IMMUNIZATION ; VACCINATION ; MODEL ; IMMUNOTHERAPY
英文摘要:

Objective. Colorectal cancer (CRC) is one of the most common malignancies in the western world. More than 60% among patients will develop liver metastases. Although surgical resection is the first choice worldwide, at this point an effective approach for the treatment of patients with liver metastasis and cancer recurrence postoperation has not yet been found. The aim of this study is to investigate the role of the allogeneic dendritomas from fusion of DCs and metastatic colon cancer cells in the activation of anti-tumor immunity against colorectal cancer liver metastases. Material and methods. Hybrids were generated by fused allogeneic human peripheral blood dendritic cells with metastatic colon cancer SW620 cells using 50% polyethylene glycol (PEG). Induction of immune responses was assessed by ex vivo ELISPOT assays. A murine model of CRC liver metastasis was used by intrasplenic injection. The validity of the vaccine was observed by Vaccination CRC liver metastasis murine model with DC/tumor hybrids. Results. The hybrids highly express the major molecules of DCs and tumor cells. The number of hybrids pulsed CTL secreting IFN-gamma was significantly higher when compared to the DC controls (p < 0.01). In a therapeutic setting mice vaccinated with in vitro cultured hybrids produced strong cellular immune responses and significant inhibition of tumor growth, compared to sham vaccinated controls. Conclusions. Vaccination with hybrids can induces strong cellular responses and significant protection from challenge in SCID mouse metastatic CRC model.

语种: 英语
所属项目编号: r0204003040531
项目资助者: Beijing Municipal Science &amp ; Technology Commission
WOS记录号: WOS:000277365200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58530
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Surg Oncol Lab, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Peking Univ, Hosp 3, Dept Intens Care Unit, Beijing 100044, Peoples R China

Recommended Citation:
Xu, Feng,Ye, Ying-Jiang,Liu, Wei,et al. Dendritic cell/tumor hybrids enhances therapeutic efficacy against colorectal cancer liver metastasis in SCID mice[J]. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY,2010,45(6):707-713.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, Feng]'s Articles
[Ye, Ying-Jiang]'s Articles
[Liu, Wei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, Feng]‘s Articles
[Ye, Ying-Jiang]‘s Articles
[Liu, Wei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace