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学科主题临床医学
Sinomenine inhibits activation of rat retinal microglia induced by advanced glycation end products
Wang, Ai Ling; Li, Zhengbin; Yuan, Ming; Yu, Albert C. H.; Zhu, Xiu′ An; Tso, Mark O. M.
关键词microglia retina AGEs sinomenine
刊名INTERNATIONAL IMMUNOPHARMACOLOGY
2007-12-05
DOI10.1016/j.intimp.2007.07.030
7期:12页:1552-1558
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Pharmacology & Pharmacy
研究领域[WOS]Immunology ; Pharmacology & Pharmacy
关键词[WOS]DIABETIC-RETINOPATHY ; ALKALOID SINOMENINE ; CYTOKINE EXPRESSION ; NITRIC-OXIDE ; MINOCYCLINE ; CULTURE ; MODEL ; MICE
英文摘要

Diabetic retinopathy involves an inflammatory response in the retina characterized by an increase in inflammatory cytokines and activation of microglia. The degree of microglia activation may influence the extent of retina injury following an inflammatory stimulus. Cytokines, released by activated microglia, regulate the influx of inflammatory cells to the damaged area. Thus, therapeutic strategy to reduce cytokine expression in microglia would be neuroprotective. Sinomenine, an alkaloid isolated from the stem and root of Sinomenium acutum, has long been recognized as an anti-inflammatory drug for rheumatoid arthritis and also inhibits macrophage activation. In this study, we activated retinal microglia in culture with advanced glycation end products (AGEs) treatment and attempted to determine whether sinomenine could reduce the production of cytokines from the activated microglia at both gene and protein levels. Changes in inflammatory cytokines, TNF alpha, IL-1 beta and IL-6, were measured by semi-quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) both in the presence and absence of AGEs. The effect of sinomenine on levels of reactive oxygen species (ROS) and the nuclear translocation of NF-kappa B p65 were studied with a laser confocal scanning microscope. AGEs treatment induced a significant release of TNF alpha, IL-l beta, and IL-6 from retinal microglia. Sinomenine could inhibit release of these cytokines. Sinomenine attenuated ROS production in a dose-dependent fashion and reduced the nuclear translocation of NF-kB p65 in AGEs-activated retinal microglia in culture. (C) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000250590000007
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58531
专题北京大学第三临床医学院_眼科
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Northwestern Univ, Dept Ophthalmol, Chicago, IL 60611 USA
3.Peking Univ, Peking Univ Eye Ctr, Peking Univ Hosp 3, Beijing 100083, Peoples R China
4.Washington Univ, Dept Mol Biol & Pharmacol, St Louis, MO 63108 USA
5.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Minist Educ, PKU, Beijing 100083, Peoples R China
7.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21287 USA
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GB/T 7714
Wang, Ai Ling,Li, Zhengbin,Yuan, Ming,et al. Sinomenine inhibits activation of rat retinal microglia induced by advanced glycation end products[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2007,7(12):1552-1558.
APA Wang, Ai Ling.,Li, Zhengbin.,Yuan, Ming.,Yu, Albert C. H..,Zhu, Xiu&prime.,...&Tso, Mark O. M..(2007).Sinomenine inhibits activation of rat retinal microglia induced by advanced glycation end products.INTERNATIONAL IMMUNOPHARMACOLOGY,7(12),1552-1558.
MLA Wang, Ai Ling,et al."Sinomenine inhibits activation of rat retinal microglia induced by advanced glycation end products".INTERNATIONAL IMMUNOPHARMACOLOGY 7.12(2007):1552-1558.
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