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学科主题: 基础医学
题名:
Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice
作者: Wang, XY; Xing, H; He, QH; Xu, JL; Wu, BJ
关键词: laser confocal scanning microscope ; brain ischemia-hypoxia injury ; lipid peroxide ; 2, 7-dichlorofluorescin diacetate
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2000
卷: 45, 期:1, 页:49-51
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM
英文摘要:

The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (PI). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.

语种: 英语
WOS记录号: WOS:000084526400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58536
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Biophys, Beijing 100083, Peoples R China
2.Beijing Med Univ, Analyt Ctr Medicopharm, Beijing 100083, Peoples R China

Recommended Citation:
Wang, XY,Xing, H,He, QH,et al. Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice[J]. CHINESE SCIENCE BULLETIN,2000,45(1):49-51.
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