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学科主题药学
SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS
Chen, Wenqian; Zhang, Yanqing2; Xie, Junbo2; Zhou, Tianyan; Lu, Wei1
关键词ferulic acid Guanxin II prescription HPLC-MS/MS paeoniflorin protocatechuic acid protocatechuic aldehyde salvianolic acid B tanshinone IIA
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2012
DOI10.1080/10826076.2011.608233
35期:6页:757-767
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; HPLC ; MEDICINE ; IDENTIFICATION ; PAEONIFLORIN ; CHEMOMETRICS ; FINGERPRINTS
英文摘要

An HPLC method coupled with triple quadrupole mass spectrometry (MS/MS) was applied to the determination of tanshinone IIA (TanllA), salvianolic acid B (SA-B), protocatechuic acid (PA), protocatechuic aldehyde (PAH), paeoniflorin (PF), and ferulic acid (FA) in Guanxinll prescription. These six compounds were analyzed simultaneously with YMC ODS-AQ column by using an aqueous mobile phase (containing 0.1% acetic acid) modified by methanol in a gradient program. The flow rate was 0.30 mL/min. Triple quadrupole mass spectrometry was optimized in both positive and negative ion multiple reaction monitoring modes for the simultaneous quantitative analysis of the two different types of active components by using a time-segment program. The method gave recoveries of 98.9-102.5% with relative standard deviations of 1.14-2.09% for the spiked herb samples. Limits of detection (LODs) ranged from 0.21 to 4.28 ng/mL and all the compounds showed good linearity in a relatively wide concentration range. The results indicated that HPLC-MS/MS provided rapid and sensitive analysis of six bioactive markers in GuanxinII prescription for quality control and authentication.

语种英语
WOS记录号WOS:000304301600002
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58542
专题北京大学药学院_药剂学系
作者单位1.Tianjin Univ Commerce, Dept Pharmaceut Engn, Tianjin, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Chen, Wenqian,Zhang, Yanqing,Xie, Junbo,et al. SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(6):757-767.
APA Chen, Wenqian,Zhang, Yanqing,Xie, Junbo,Zhou, Tianyan,&Lu, Wei.(2012).SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,35(6),757-767.
MLA Chen, Wenqian,et al."SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35.6(2012):757-767.
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