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学科主题: 药学
题名:
SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS
作者: Chen, Wenqian; Zhang, Yanqing2; Xie, Junbo2; Zhou, Tianyan; Lu, Wei1
关键词: ferulic acid ; Guanxin II prescription ; HPLC-MS/MS ; paeoniflorin ; protocatechuic acid ; protocatechuic aldehyde ; salvianolic acid B ; tanshinone IIA
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2012
DOI: 10.1080/10826076.2011.608233
卷: 35, 期:6, 页:757-767
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: MASS-SPECTROMETRY ; HPLC ; MEDICINE ; IDENTIFICATION ; PAEONIFLORIN ; CHEMOMETRICS ; FINGERPRINTS
英文摘要:

An HPLC method coupled with triple quadrupole mass spectrometry (MS/MS) was applied to the determination of tanshinone IIA (TanllA), salvianolic acid B (SA-B), protocatechuic acid (PA), protocatechuic aldehyde (PAH), paeoniflorin (PF), and ferulic acid (FA) in Guanxinll prescription. These six compounds were analyzed simultaneously with YMC ODS-AQ column by using an aqueous mobile phase (containing 0.1% acetic acid) modified by methanol in a gradient program. The flow rate was 0.30 mL/min. Triple quadrupole mass spectrometry was optimized in both positive and negative ion multiple reaction monitoring modes for the simultaneous quantitative analysis of the two different types of active components by using a time-segment program. The method gave recoveries of 98.9-102.5% with relative standard deviations of 1.14-2.09% for the spiked herb samples. Limits of detection (LODs) ranged from 0.21 to 4.28 ng/mL and all the compounds showed good linearity in a relatively wide concentration range. The results indicated that HPLC-MS/MS provided rapid and sensitive analysis of six bioactive markers in GuanxinII prescription for quality control and authentication.

语种: 英语
WOS记录号: WOS:000304301600002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58542
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Tianjin Univ Commerce, Dept Pharmaceut Engn, Tianjin, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Wenqian,Zhang, Yanqing,Xie, Junbo,et al. SIMULTANEOUS DETERMINATION OF SIX PHENOLIC COMPOUNDS IN GUANXIN II PRESCRIPTION BY HPLC/MS/MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(6):757-767.
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