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学科主题基础医学
Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold
Xia, Yang1,2; Mei, Fang1; Duan, Yongli2; Gao, Ying2; Xiong, Zhuo3; Zhang, Ting3; Zhang, Hongquan1
关键词sodium alginate gelatin BMSCs osteogenic cells ectopic bone formation calvarial critical-sized defects
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2012-04-01
DOI10.1002/jbm.a.33232
100A期:4页:1044-1050
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MESENCHYMAL STEM-CELLS ; CRITICAL SIZE DEFECT ; FABRICATION ; HYDROGELS ; MODEL
英文摘要

To investigate the potential application of bone marrow stromal cells (BMSCs) and an injectable sodium alginate/gelatin scaffold for bone tissue engineering (BTE). The phenotype of osteogenic BMSCs was examined by mineralized nodules formation and type I collagen expression. Cell proliferation was evaluated by MTT assay. The biocompatibility of scaffold and osteogenic cells were examined by hematoxylin and eosin (H&E) staining. Ectopic bone formation as well as closure of rabbit calvarial critical-sized defects following scaffold-cell implantation were analyzed by histological examination and computed tomography (CT) scanning. Spindle-shaped osteogenic cells of high purity were derived from BMSCs. The osteogenic cells and sodium alginate/gelatin (2:3) scaffold presented fine biocompatibility following cross-linking with 0.6% of CaCl2. After implantation, the scaffold-cell construct promoted both ectopic bone formation and bone healing in the rabbit calvarial critical-sized defect model. Our data demonstrated that the sodium alginate/gelatin scaffold could be a suitable biomaterial for bone engineering, and the scaffold-osteogenic cells construct is a promising alternative approach for the bone healing process. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.

语种英语
WOS记录号WOS:000300677600024
项目编号7083107 ; 20071D0303100116 ; 20090460179 ; 30830048 ; 2010CB912203
资助机构Beijing Natural Science Foundation ; Beijing Excellent Researcher Foundation ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; State Key Development Program of Basic Research of China
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58544
专题北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Beijing Shijitan Hosp, Beijing 100038, Peoples R China
3.Tsinghua Univ, Dept Mech Engn, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714
Xia, Yang,Mei, Fang,Duan, Yongli,et al. Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2012,100A(4):1044-1050.
APA Xia, Yang.,Mei, Fang.,Duan, Yongli.,Gao, Ying.,Xiong, Zhuo.,...&Zhang, Hongquan.(2012).Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,100A(4),1044-1050.
MLA Xia, Yang,et al."Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 100A.4(2012):1044-1050.
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