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Haemoglobin A1c measurement in patients with chronic kidney disease
Li, Qing1; Ju, Yi1; Jin, Ting2; Pang, Biqiong2; Deng, Jiade3; Du, Tongxin4; Wang, Hong5
关键词HbA(1c) Carbamylated haemoglobin Ion-exchange chromatography Affinity chromatography
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology

Objectives: The aim of this study is to investigate the interference of carbamylated haemoglobin (cHb) to haemoglobin A(1c) (HbA(1c)) measurements in patients with chronic kidney disease (CKD) by three ion-exchange chromatography methods.

Design and methods: We included three ion-exchange chromatography methods (Bio-Rad Variant II, G7 and HA-8160) and one affinity chromatography method (Primus Ultra2) to test HbA(1c). In vitro carbamylation of haemoglobin was also performed to test interference of carbamylation.

Results: 152 CKD patients were diagnosed. The average HbA(1c) and urea levels of nondiabetic and diabetic patients were 5.24% (33.8 mmol/mol) and 7.36% (56.9 mmol/mol) and 16.2 mmol/L and 11.2 mmol/L, respectively. Repeated measures ANOVA showed that the difference of HbA(1c) values between four methods is not statistically significant (P = 0.421 for non-DM and P = 0.336 for DM). With the increase in blood urea concentration from stage 3 to stage 5, there was no corresponding increase in HbA(1c) values for both groups. In vitro carbamylation results showed that carbamylated Hb peaks could be clearly identified by the three ion-exchange methods.

Conclusion: HbA(1c) measurement by these methods may not be impacted by carbamylated Hb. The results should be interpreted with caution in high carbamylated Hb patients. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

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作者单位1.Shanghai Ctr Clin Lab, Shanghai 200126, Peoples R China
2.First Hosp Fuzhou, Fuzhou, Fujian Province, Peoples R China
3.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
4.First Municipal Peoples Hosp Guangzhou, Guangzhou, Guangdong, Peoples R China
5.Nanjing Med Univ, Nanjing Clin Nucl Med Ctr, Nanjing Hosp 1, Nanjing, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Li, Qing,Ju, Yi,Jin, Ting,et al. Haemoglobin A1c measurement in patients with chronic kidney disease[J]. CLINICAL BIOCHEMISTRY,2014,47(6):481-484.
APA Li, Qing.,Ju, Yi.,Jin, Ting.,Pang, Biqiong.,Deng, Jiade.,...&Wang, Hong.(2014).Haemoglobin A1c measurement in patients with chronic kidney disease.CLINICAL BIOCHEMISTRY,47(6),481-484.
MLA Li, Qing,et al."Haemoglobin A1c measurement in patients with chronic kidney disease".CLINICAL BIOCHEMISTRY 47.6(2014):481-484.
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