IR@PKUHSC  > 北京大学药学院
学科主题药学
The effect of hydrophilic and hydrophobic structure of amphiphilic polymeric micelles on their transport in epithelial MDCK cells
Yu, Chao1; He, Bing1; Xiong, Meng-Hua2,3; Zhang, Hua1; Yuan, Lan4; Ma, Ling1; Dai, Wen-Bing1; Wang, Jun2,3; Wang, Xing-Lin5; Wang, Xue-Qing1; Zhang, Qiang1
关键词Hydrophilicity Hydrophobicity Amphiphilic micelles Transport Epithelial cells
刊名BIOMATERIALS
2013-08-01
DOI10.1016/j.biomaterials.2013.05.006
34期:26页:6284-6298
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]DRUG-DELIVERY ; INTRACELLULAR TRAFFICKING ; UPTAKE MECHANISMS ; CELLULAR UPTAKE ; NANOPARTICLES ; ENDOCYTOSIS ; NANOCARRIERS ; EXOCYTOSIS ; TOXICITY ; OPPORTUNITIES
英文摘要

The interaction of nanocarriers with cells including their transcellular behavior is vital not only for a drug delivery system, but also for the safety of nanomaterials. In an attempt to clarify how the structures of polymers impact the transport mechanisms of their nanocarriers in epithelial cells, three amphiphilic polymers (PEEP-PCL, PEG-PCL and PEG-DSPE) with different hydrophilic or hydrophobic blocks were synthesized or chosen to form different micelle systems here. The endocytosis, exocytosis, intracellular colocalization, paracellular permeability and transcytosis of these micelle systems were compared using Forster resonance energy transfer analysis, real-time confocal images, colocalization assay, trans-epithelial electrical resistance study, and so on. All micelle systems were found intact during the studies with cells. The endocytosis and exocytosis studies with undifferentiated MDCK cells and the transcytosis study with differentiated MDCK monolayers all indicated the fact that PEG-DSPE micelles achieved the most and fastest transport, followed by PEG-PCL and PEEP-PCL in order. These might be because DSPE has higher hydrophobicity than PCL while PEG has lower hydrophilicity than PEEP. Different in hydrophilic or hydrophobic structures, all kinds of micelles demonstrated similar pathways during endocytosis and exocytosis, both caveolae- and clathrin-mediated but with difference in degree. The colocalization studies revealed different behaviors in intracellular trafficking among the three polymer micelles, suggesting the decisive role of hydrophilic shells on this process. Finally, all micelle systems did not impact the paracellular permeability of test cell monolayer. In conclusion, the hydrophilic and hydrophobic structures of test micelles could influence their transport ability, intracellular trafficking and the transport level under each pathway in MDCK cells. (C) 2013 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000321079600019
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58551
Collection北京大学药学院
北京大学医学信息学中心
北京大学基础医学院
北京大学药学院_药剂学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
4.Peking Univ, Med & Healthy Analyt Ctr, Beijing 100191, Peoples R China
5.Tianjin Inst Pharmaceut Res, State Key Lab Drug Delivery Technol & Phannacokin, Tianjin 300193, Peoples R China
Recommended Citation
GB/T 7714
Yu, Chao,He, Bing,Xiong, Meng-Hua,et al. The effect of hydrophilic and hydrophobic structure of amphiphilic polymeric micelles on their transport in epithelial MDCK cells[J]. BIOMATERIALS,2013,34(26):6284-6298.
APA Yu, Chao.,He, Bing.,Xiong, Meng-Hua.,Zhang, Hua.,Yuan, Lan.,...&Zhang, Qiang.(2013).The effect of hydrophilic and hydrophobic structure of amphiphilic polymeric micelles on their transport in epithelial MDCK cells.BIOMATERIALS,34(26),6284-6298.
MLA Yu, Chao,et al."The effect of hydrophilic and hydrophobic structure of amphiphilic polymeric micelles on their transport in epithelial MDCK cells".BIOMATERIALS 34.26(2013):6284-6298.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yu, Chao]'s Articles
[He, Bing]'s Articles
[Xiong, Meng-Hua]'s Articles
百度学术
百度学术Similar articles in
[Yu, Chao]'s Articles
[He, Bing]'s Articles
[Xiong, Meng-Hua]'s Articles
必应学术
必应学术Similar articles in
[Yu, Chao]'s Articles
[He, Bing]'s Articles
[Xiong, Meng-Hua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.