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学科主题: 临床医学
题名:
Preparation of humanized ovarian carcinoma anti-idiotypic minibody
作者: Chang, XH; Cui, H; Feng, J; Li, Y; Liu, B; Cao, SJ; Cheng, YX; Qian, HN
刊名: HYBRIDOMA AND HYBRIDOMICS
发表日期: 2003-04-01
卷: 22, 期:2, 页:109-115
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: MONOCLONAL ANTIIDIOTYPIC ANTIBODY ; SINGLE-CHAIN FRAGMENT ; IMMUNOLOGICAL CONSOLIDATION ; PALLIATIVE TREATMENT ; IMMUNE-RESPONSES ; VACCINE ACA125 ; CANCER ; IMMUNOTHERAPY ; SURVIVAL ; PROTEIN
英文摘要:

Murine anti-idiotypic monoclonal antibody (MAb) 6B11 mimicking the tumor-associated antigen OC166-9 is used as a vaccine for the induction of an anti-tumoral immunity in experiments of in vitro and in vivo animal model with ovarian carcinoma. In this article, we have humanized 6B11 anti-idiotypic minibody using overlap polymerase chain reaction (PCR) and DNA recombinant technique, prokaryotic expression vector was produced by genetic fusion of 6B11V(L)-V-H to human IgG1 hinge and CH3 region. Transformed E. coli BL21(DE3) were propagated and induced by isopropyl-beta D-thiogalactopyranoside (IPTG). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that a protein band with molecular weight of 50kD appeared as the expected size after transformation. Molecular weight of 100 kDa may be examined by electrophoresis in nondenaturing systems. The fusion protein was analyzed with enzyme-linked immunosorbant assay (ELISA), inhibition ELISA tests and Western blot, respectively. The humanized anti-idiotype minibody showed capacity of bivalent binding to ovarian cancer MAb COC166-9 and goat antihuman immunoglobulin IgG1. It is useful reagents for clinical use.

语种: 英语
WOS记录号: WOS:000183482000006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58564
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Chang, XH,Cui, H,Feng, J,et al. Preparation of humanized ovarian carcinoma anti-idiotypic minibody[J]. HYBRIDOMA AND HYBRIDOMICS,2003,22(2):109-115.
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