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学科主题基础医学
Adenovirus-mediated CTLA4-FasL gene transfer prevents autoimmune diabetes in mice induced by multiple low doses of streptozotocin
Jin, YZ; Wang, GM; Li, AL; Hao, J; Gao, X; Xie, SS
关键词insulin-dependent diabetes mellitus adenoviral vectors streptozotocin gene therapy apoptosis
刊名CHINESE SCIENCE BULLETIN
2004-03-01
DOI10.1360/03wc0532
49期:5页:476-481
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]FAS LIGAND ; EXPRESSION ; MELLITUS ; CELLS
英文摘要

Type 1 diabetes is the result of a selective destruction of insulin-producing P cells in pancreatic islets by autoreactive T cells. Depletion of autoreactive T cell through apoptosis may be a potential strategy for the prevention of autoimmune diabetes. Simultaneous stimulation of Fas-mediated pathway and blockade of costimulation by a CTLA4-FasL fusion protein has been reported to lead to substantial inhibition of mixed lymphocyte reaction and enhanced in vitro apoptosis of peripheral lymphocytes. To test the feasibility of CTLA4-FasL- based gene therapy to prevent autoimmune diabetes, we developed recombinant adenovirus containing human C7LA4-FasL gene (Ad-CTLA4-FasL). A single injection of 2X10(8) plaque forming units (PFU) of AdCTLA4-FasL via tail vein dramatically reduced the incidence of autoimmune diabetes in mice induced by multiple low doses of streptozotocin. AdCTLA4-FasL administration maintained islet insulin content, significantly increased apoptosis of pancreatic lymphocytes, quantitatively reduced IFN-gamma and Vbeta8.2 TCR chain mRNA expression in pancreatic lymphocytes. These results indicate the therapeutic potential of simultaneous stimulation of Fas-mediated pathway and blockade of costimulation by adenovirus-mediated CTLA4-FasL gene transfer in the prevention of autoimmune diabetes.

语种英语
WOS记录号WOS:000221118900012
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58585
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Ctr Hlth Sci, Dept Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Jin, YZ,Wang, GM,Li, AL,et al. Adenovirus-mediated CTLA4-FasL gene transfer prevents autoimmune diabetes in mice induced by multiple low doses of streptozotocin[J]. CHINESE SCIENCE BULLETIN,2004,49(5):476-481.
APA Jin, YZ,Wang, GM,Li, AL,Hao, J,Gao, X,&Xie, SS.(2004).Adenovirus-mediated CTLA4-FasL gene transfer prevents autoimmune diabetes in mice induced by multiple low doses of streptozotocin.CHINESE SCIENCE BULLETIN,49(5),476-481.
MLA Jin, YZ,et al."Adenovirus-mediated CTLA4-FasL gene transfer prevents autoimmune diabetes in mice induced by multiple low doses of streptozotocin".CHINESE SCIENCE BULLETIN 49.5(2004):476-481.
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