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学科主题: 临床医学
题名:
Amplification and specific expression of T-bet gene in nasal NK/T-cell lymphoma
作者: Ye, Y.; Li, T.; Zhang, B.; Guo, Z.
关键词: Nasal NK/T-cell lymphoma ; RLGS ; T-bet
刊名: LEUKEMIA & LYMPHOMA
发表日期: 2007
DOI: 10.1080/10428190600955902
卷: 48, 期:1, 页:168-173
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Hematology
研究领域[WOS]: Oncology ; Hematology
关键词[WOS]: V-ALPHA-14I NKT CELLS ; GENOME-WIDE SCAN ; TRANSCRIPTION FACTOR ; C-KIT ; MUTATIONS ; PREECLAMPSIA ; MATURATION ; LEUKEMIA ; JAPAN ; P53
英文摘要:

To define the specific genetic alterations in nasal natural killer/T-cell lymphoma (N-NK/T-L), the assay of restriction landmark genomic scanning (RLGS), a genome-wide method, was used to investigate a pair of genomic DNA from N-NK/T-L cells and peripheral blood leukocytes of the same patient. The intensified spots in displayed N-NK/T-L gel of RLGS were subjected to bioinformatic analysis by virtual genome scan (VGS) and one of candidate spots proved to be T-bet gene (T-box expressed in T-cell). The pair of samples was consequently analysed by Southern hybridization, revealing genomic amplification of T-bet but not different status of DNA methylation. The amplification of T-bet was also discovered in other cases of N-NK/T-L by dot blotting. The expression of T-bet was investigated by in situ hybridization (ISH) and immunohistochemistry (IHC). The results showed that T-bet predominantly expressed in N-NK/T-L (ISH: 90.0%, 18/20; IHC: 80%, 16/20), while few cases of B-cell lymphoma (ISH: 11.8%, 2/17; IHC: 17.6%, 3/17) or T-cell lymphoma (ISH: 33.3%, 2/6; IHC: 16.7%, 1/6) were positive. The difference of T-bet expression in either ISH or IHC between N-NK/T-L and B-cell lymphoma or T-cell lymphoma was statistically significant (P < 0.05). Moreover, the expression of T-bet was not detected in normal spleen tissue and chronic inflammatory nasal mucosa. The results suggested that the amplification of T-bet gene or resulting its over-expression of T-bet gene might be involved in the development of N-NK/T-L and thus it should be worth confirming whether the over-expression of T-bet be helpful to the diagnosis of N-NK/T-L in further study.

语种: 英语
WOS记录号: WOS:000244249900023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58597
Appears in Collections:北京大学第一临床医学院_病理科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pathol, Beijing, Peoples R China
2.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Ye, Y.,Li, T.,Zhang, B.,et al. Amplification and specific expression of T-bet gene in nasal NK/T-cell lymphoma[J]. LEUKEMIA &amp; LYMPHOMA,2007,48(1):168-173.
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