北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Marsdenia tenacissima extract restored gefitinib sensitivity in resistant non-small cell lung cancer cells
作者: Han, Shu-Yan1; Zhao, Ming-Bo2; Zhuang, Gui-Bao1; Li, Ping-Ping1
关键词: Marsdenia tenacissima extract ; Gefitinib resistance ; NSCLC ; T790M ; K-ras mutations
刊名: LUNG CANCER
发表日期: 2012
DOI: 10.1016/j.lungcan.2011.06.001
卷: 75, 期:1, 页:30-37
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Respiratory System
研究领域[WOS]: Oncology ; Respiratory System
关键词[WOS]: GROWTH-FACTOR-RECEPTOR ; TYROSINE KINASE INHIBITORS ; MEDIATED MULTIDRUG-RESISTANCE ; MET AMPLIFICATION ; ACQUIRED-RESISTANCE ; T790M MUTATION ; EGFR T790M ; POLYOXYPREGNANE GLYCOSIDES ; 1ST-LINE GEFITINIB ; THERAPY
英文摘要:

Most non-small cell lung cancer (NSCLC) patients responding to gefitinib harbor activating mutations in the epidermal growth factor receptor (EGFR). However, the responsive cases eventually develop the resistance to gefitinib. Besides, K-ras mutations were identified as the primary resistance to gefitinib. We investigated whether Marsdenia tenacissima extract (MTE, trade name: Xiao-Ai-Ping injection) combined with gefitinib could overcome the resistance of NSCLC cells to gefitinib. NSCLC cell lines with different sensitivities to gefitinib were studied. Cell growth and apoptosis were evaluated by MTT assay and flow cytometry, respectively. The EGFR-related signaling molecule phosphorylation was assessed by Western blotting. We found that MTE inhibited cell growth in gefitinib-sensitive and -resistant cells. In gefitinib-resistant cells, the MTE -> MTE + gefitinib (M -> M + G) treatment was most potent over the concurrent administration of MTE and gefitinib (M + G) or gefitinib -> gefitinib + MTE (G -> G + M) treatment in cell growth inhibition and apoptosis induction. The M -> M + G treatment significantly reduced the phosphorylation of EGFR downstream signaling molecules PI3K/Akt/mTOR and ERK, on which MTE and gefitinib alone had no obvious effects on the resistant cells. The M -> M + G treatment attenuated c-Met phosphorylation in H460 and H1975 as well. Thus, we found that the M -> M + G treatment improved the sensitivity of resistant NSCLC cells carrying 1790 M or K-ras mutations to gefitinib, suggesting that the M -> M + G treatment may be a promising therapeutic strategy to overcome gefitinib resistance in NSCLC. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 20090450009 ; 7112027
项目资助者: China Postdoctoral Science Foundation ; Beijing Natural Science Foundation
WOS记录号: WOS:000299450600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58608
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Integrat Chinese & Western Med, Sch Oncol,Beijing Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Han, Shu-Yan,Zhao, Ming-Bo,Zhuang, Gui-Bao,et al. Marsdenia tenacissima extract restored gefitinib sensitivity in resistant non-small cell lung cancer cells[J]. LUNG CANCER,2012,75(1):30-37.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Han, Shu-Yan]'s Articles
[Zhao, Ming-Bo]'s Articles
[Zhuang, Gui-Bao]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Han, Shu-Yan]‘s Articles
[Zhao, Ming-Bo]‘s Articles
[Zhuang, Gui-Bao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace