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On-line study of flavonoids of Trollius chinensis Bunge binding to DNA with ethidium bromide using a novel combination of chromatographic, mass spectrometric and fluorescence techniques
Song, Zhiling1; Wang, Hong1; Ren, Biao1; Zhang, Baobao1; Hashi, Yuki2; Chen, Shizhong1
关键词Trollius chinensis Bunge Flavonoids DNA binders HPLC-DAD-ESI-IT-TOF-MS-DNA-EB-FLD
刊名JOURNAL OF CHROMATOGRAPHY A
2013-03-22
DOI10.1016/j.chroma.2013.01.060
1282页:102-112
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者Peking University ; Shimadzu International Trading (Shanghai) Co. Limited ; Peking University ; Shimadzu International Trading (Shanghai) Co. Limited
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]C-GLYCOSIDES ; LIQUID-CHROMATOGRAPHY ; IDENTIFICATION ; LEDEBOURI ; INHIBITORS ; FLOWERS ; PROBE
英文摘要

The study of the interaction between drugs and DNA is an important way to understand the role of drug molecules. A novel online analytical method for this purpose combining high-performance liquid chromatography-diode array detector-electrospray ionization-ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MSn) and DNA-ethidium bromide detection with a fluorescence detector (DNA-EB-FLD) was firstly developed, which could rapidly identify the chemical constituents and obtain the profile related to DNA binding activity. This method has been applied for a precise or probable identification of the chemical constituents by ultraviolet (UV) absorption and MSn data analysis, while the DNA binding profile has been characterized by directly measuring the fluorescence intensity of compound-DNA-EB. Using this method, Trollius chinensis Bunge was studied and 18 constituents were identified by MSn data; six of them (4′-methoxy-2 ′′-0-(2′′′-methylbutyryl)vitexin,2 ′′-0-(3"′-methoxycaffeoyl)vitexin) and 4′-methoxy-2 ′′-0-(2"′-methylbutyryl)orientin,acacetin-7-0-rutinoside,quercetin-3-0xylosylglucoside,quercetin-3-0-arabinosylglucoside) were identified for the first time in T. chinensis Bunge, and 16 constituents accounted for its activity of binding to DNA. The established (HPLC-DAD-ESI-IT-TOF-MSn DNA-EB-FLD) system has proved to offer a useful strategy for correlating the chemical profile with the binding to DNA activities of the components without their isolation and purification, and may be used for multicomponent analysis of active substances in other herbs. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2009ZX09301-010
资助者Peking University ; Shimadzu International Trading (Shanghai) Co. Limited ; Peking University ; Shimadzu International Trading (Shanghai) Co. Limited
WOS记录号WOS:000315832000011
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58636
专题北京大学药学院
作者单位1.Shimadzu Int Trading Shanghai Co Ltd, Beijing 100020, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Song, Zhiling,Wang, Hong,Ren, Biao,et al. On-line study of flavonoids of Trollius chinensis Bunge binding to DNA with ethidium bromide using a novel combination of chromatographic, mass spectrometric and fluorescence techniques[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1282:102-112.
APA Song, Zhiling,Wang, Hong,Ren, Biao,Zhang, Baobao,Hashi, Yuki,&Chen, Shizhong.(2013).On-line study of flavonoids of Trollius chinensis Bunge binding to DNA with ethidium bromide using a novel combination of chromatographic, mass spectrometric and fluorescence techniques.JOURNAL OF CHROMATOGRAPHY A,1282,102-112.
MLA Song, Zhiling,et al."On-line study of flavonoids of Trollius chinensis Bunge binding to DNA with ethidium bromide using a novel combination of chromatographic, mass spectrometric and fluorescence techniques".JOURNAL OF CHROMATOGRAPHY A 1282(2013):102-112.
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