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Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells
Chen, CP; Li, XX; Zhang, LR; Min, JM; Chan, JYW; Fung, KP; Wang, SQ; Zhang, LH
刊名BIOCONJUGATE CHEMISTRY
2002-05-01
DOI10.1021/bc015540f
13期:3页:525-529
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SOLID-PHASE SYNTHESIS ; SIGNAL SEQUENCE ; NUCLEOPEPTIDE FRAGMENT ; FACILE PREPARATION ; MODEL MEMBRANES ; MESSENGER-RNA ; HYBRIDS ; OLIGODEOXYNUCLEOTIDES ; INHIBITION ; INCREASES
英文摘要

A simple procedure for the preparation of oligonucleotide-peptide conjugate was developed. p-Hydroxybenzoic acid was used as a linker for the connection of the fragments of peptide and oligonucleotide. It was found that such formed linkage was stable under the conditions of conjugate synthesis. The designed conjugate targeting to GLUT-1 showed up to 50% inhibition of cell proliferation in HepG-2 and MCF-7 cells. Comparing to the results from the expressed antisense RNA in cancer cells, it was proposed that the conjugate of signal peptide mimic and antisense oligonucleotide could improve the permeability of antisense oligonucleotide through cell membrane.

语种英语
WOS记录号WOS:000175684800019
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58640
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
推荐引用方式
GB/T 7714
Chen, CP,Li, XX,Zhang, LR,et al. Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells[J]. BIOCONJUGATE CHEMISTRY,2002,13(3):525-529.
APA Chen, CP.,Li, XX.,Zhang, LR.,Min, JM.,Chan, JYW.,...&Zhang, LH.(2002).Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells.BIOCONJUGATE CHEMISTRY,13(3),525-529.
MLA Chen, CP,et al."Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells".BIOCONJUGATE CHEMISTRY 13.3(2002):525-529.
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