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学科主题: 药学
题名:
Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells
作者: Chen, CP; Li, XX; Zhang, LR; Min, JM; Chan, JYW; Fung, KP; Wang, SQ; Zhang, LH
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2002-05-01
DOI: 10.1021/bc015540f
卷: 13, 期:3, 页:525-529
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: SOLID-PHASE SYNTHESIS ; SIGNAL SEQUENCE ; NUCLEOPEPTIDE FRAGMENT ; FACILE PREPARATION ; MODEL MEMBRANES ; MESSENGER-RNA ; HYBRIDS ; OLIGODEOXYNUCLEOTIDES ; INHIBITION ; INCREASES
英文摘要:

A simple procedure for the preparation of oligonucleotide-peptide conjugate was developed. p-Hydroxybenzoic acid was used as a linker for the connection of the fragments of peptide and oligonucleotide. It was found that such formed linkage was stable under the conditions of conjugate synthesis. The designed conjugate targeting to GLUT-1 showed up to 50% inhibition of cell proliferation in HepG-2 and MCF-7 cells. Comparing to the results from the expressed antisense RNA in cancer cells, it was proposed that the conjugate of signal peptide mimic and antisense oligonucleotide could improve the permeability of antisense oligonucleotide through cell membrane.

语种: 英语
WOS记录号: WOS:000175684800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58640
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China

Recommended Citation:
Chen, CP,Li, XX,Zhang, LR,et al. Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells[J]. BIOCONJUGATE CHEMISTRY,2002,13(3):525-529.
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