IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation
Fu Wei-li1; Jia Zhu-qing2; Wang Wei-ping2; Zhang Ji-ying1; Fu Xin1; Duan Xiao-ning1; Leung Kevin Kar Ming1; Zhou Chun-yan2; Yu Jia-kuo1
关键词mesenchymal stem cells derived from peripheral blood hypoxia serum deprivation proliferation apoptosis
刊名CHINESE MEDICAL JOURNAL
2011-12-05
DOI10.3760/cma.j.issn.0366-6999.2011.23.022
124期:23页:3959-3967
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation
研究领域[WOS]General & Internal Medicine
关键词[WOS]THERAPY POSITION STATEMENT ; INTERNATIONAL-SOCIETY ; CARTILAGE REPAIR ; CLINICAL-TRIALS ; DIFFERENTIATE ; KNEE ; VEGF
英文摘要

Background The proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood (PB-MSCs) were investigated under hypoxia and serum deprivation conditions in vitro so as to evaluate the feasibility for autologous PB-MSCs applications in cartilage repair.

Methods MSCs were mobilized into peripheral blood by granulocyte colony stimulating factor (G-CSF) and AMD3100. The blood samples were collected from central ear artery of rabbits. Adhered cells were obtained by erythrocyte lysis buffer and identified as MSCs by adherence to plastic, spindle shaped morphology, specific surface markers, differentiation abilities into osteoblasts, adipocytes and chondroblasts in vitro under appropriate conditions. MSCs were cultured in four groups at different oxygen tension (20% O-2 and 2% O-2), with or without 10% fetal bovine serum (FBS) conditions: 20% O-2 and 10% FBS complete medium (normal medium, N), 20% O-2 and serum deprivation medium (D), 2% O-2 and 10% FBS complete medium (hypoxia, H), 2% O-2 and serum deprivation (HD). Cell proliferation was determined by CCK-8 assay. Apoptosis was detected by Annexin V/PI and terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) staining.

Results Spindle-shaped adherent cells were effectively mobilized from peripheral blood by a combined administration of G-CSF plus AMD3100. These cells showed typical fibroblast-like phenotype similar to MSCs from bone marrow (BM-MSCs), and expressed a high level of typical MSCs markers CD29 and CD44, but lacked in the expression of hematopoietic markers CD45 and major histocompatibility complex Class II (MHC II). They could also differentiate into osteoblasts, adipocytes and chondroblasts in vitro under appropriate conditions. No significant morphological differences were found among the four groups. It was found that hypoxia could enhance proliferation of PB-MSCs regardless of serum concentration, but serum deprivation inhibited proliferation at the later stage of culture. Apart from that, hypoxia or serum deprivation could promote the apoptosis of PB-MSCs after 48 hours; the effect was stronger when these two conditions combined together. Furthermore, the effect of serum deprivation on apoptosis was stronger compared with that of hypoxia.

Conclusions PB-MSCs possess similar phenotypes as BM-MSCs. Their differentiation and proliferation abilities make them a new source of seed cells for ischemia-related cell therapy and tissue engineering in the field of the articular cartilage repair. Chin Med J 2011;124(23):3959-3967

语种英语
所属项目编号30471783 ; 20030001009 ; 7052046
资助者National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation
WOS记录号WOS:000299061300022
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58656
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Fu Wei-li,Jia Zhu-qing,Wang Wei-ping,et al. Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation[J]. CHINESE MEDICAL JOURNAL,2011,124(23):3959-3967.
APA Fu Wei-li.,Jia Zhu-qing.,Wang Wei-ping.,Zhang Ji-ying.,Fu Xin.,...&Yu Jia-kuo.(2011).Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation.CHINESE MEDICAL JOURNAL,124(23),3959-3967.
MLA Fu Wei-li,et al."Proliferation and apoptosis property of mesenchymal stem cells derived from peripheral blood under the culture conditions of hypoxia and serum deprivation".CHINESE MEDICAL JOURNAL 124.23(2011):3959-3967.
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