IR@PKUHSC  > 北京大学第二临床医学院  > 胃肠外科
学科主题临床医学
Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis
Zhang, Yanbin1; Ye, Yingjiang1; Shen, Danhua2; Jiang, Kewei1; Zhang, Hui1; Sun, Wei3; Zhang, Jiyang3; Xu, Feng1; Cui, Zhirong1; Wang, Shan1
刊名CANCER SCIENCE
2010-02-01
DOI10.1111/j.1349-7006.2009.01424.x
101期:2页:523-529
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]GEL-ELECTROPHORESIS ; HEPATOCELLULAR-CARCINOMA ; CELL-LINES ; EXPRESSION ; ADENOCARCINOMA ; TRANSFORMATION ; TUMORIGENESIS ; METASTASIS ; PROTEINS ; SURVIVAL
英文摘要

To search for potential protein markers of colorectal cancer (CRC), the changes in protein expression levels between microdissected tumor cells and normal mucosa epithelia were analyzed by an acetylation stable isotopic labeling method coupled with linear quadrupole ion trap fourier transform mass spectrometry (LTQ-FTMS). In total, 137 proteins were up-regulated or down-regulated significantly in cancer by at least two-fold. Based on gene ontology analysis, the largest part of differential proteins were unknown for both subcellular localization and biological process. In particular, the significant up-regulation of transgelin-2 (TAGLN2) in CRC was validated by Western blot analysis and further evaluated by immunohistochemistry in paired tumor and normal mucosa samples from 120 consecutive CRC patients, 20 adenomas, and eight synchronous hepatic metastases of CRC. TAGLN2 expression was frequently observed in cancer cells, precancerous lesions, and hepatic metastases, whereas in normal epithelia expression was rarely observed. The overexpression of TAGLN2 was associated with lymph node and distant metastasis, advanced clinical stage (P < 0.001), and shorter overall survival in CRCs. Cox regression analysis indicated that high tumor-TAGLN2 expression represents an independent prognostic factor. Consequently, over-expression of TAGLN2 may serve as a new biomarker for predicting progression and prognosis of CRC. (Cancer Sci 2010; 101: 523-529)

语种英语
WOS记录号WOS:000274156400034
项目编号2005-2010
资助机构Capital Medical Development Foundation of the Beijing Municipal Health Bureau, P.R. China
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58663
专题北京大学第二临床医学院_胃肠外科
北京大学第二临床医学院_病理科
作者单位1.Beijing Proteome Res Ctr, State Key Lab Prote, Beijing, Peoples R China
2.Peking Univ, Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing 100871, Peoples R China
3.Peking Univ, Peking Univ Peoples Hosp, Dept Pathol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yanbin,Ye, Yingjiang,Shen, Danhua,et al. Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis[J]. CANCER SCIENCE,2010,101(2):523-529.
APA Zhang, Yanbin.,Ye, Yingjiang.,Shen, Danhua.,Jiang, Kewei.,Zhang, Hui.,...&Wang, Shan.(2010).Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis.CANCER SCIENCE,101(2),523-529.
MLA Zhang, Yanbin,et al."Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis".CANCER SCIENCE 101.2(2010):523-529.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Yanbin]的文章
[Ye, Yingjiang]的文章
[Shen, Danhua]的文章
百度学术
百度学术中相似的文章
[Zhang, Yanbin]的文章
[Ye, Yingjiang]的文章
[Shen, Danhua]的文章
必应学术
必应学术中相似的文章
[Zhang, Yanbin]的文章
[Ye, Yingjiang]的文章
[Shen, Danhua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。