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学科主题: 临床医学
题名:
Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis
作者: Zhang, Yanbin1; Ye, Yingjiang1; Shen, Danhua2; Jiang, Kewei1; Zhang, Hui1; Sun, Wei3; Zhang, Jiyang3; Xu, Feng1; Cui, Zhirong1; Wang, Shan1
刊名: CANCER SCIENCE
发表日期: 2010-02-01
DOI: 10.1111/j.1349-7006.2009.01424.x
卷: 101, 期:2, 页:523-529
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GEL-ELECTROPHORESIS ; HEPATOCELLULAR-CARCINOMA ; CELL-LINES ; EXPRESSION ; ADENOCARCINOMA ; TRANSFORMATION ; TUMORIGENESIS ; METASTASIS ; PROTEINS ; SURVIVAL
英文摘要:

To search for potential protein markers of colorectal cancer (CRC), the changes in protein expression levels between microdissected tumor cells and normal mucosa epithelia were analyzed by an acetylation stable isotopic labeling method coupled with linear quadrupole ion trap fourier transform mass spectrometry (LTQ-FTMS). In total, 137 proteins were up-regulated or down-regulated significantly in cancer by at least two-fold. Based on gene ontology analysis, the largest part of differential proteins were unknown for both subcellular localization and biological process. In particular, the significant up-regulation of transgelin-2 (TAGLN2) in CRC was validated by Western blot analysis and further evaluated by immunohistochemistry in paired tumor and normal mucosa samples from 120 consecutive CRC patients, 20 adenomas, and eight synchronous hepatic metastases of CRC. TAGLN2 expression was frequently observed in cancer cells, precancerous lesions, and hepatic metastases, whereas in normal epithelia expression was rarely observed. The overexpression of TAGLN2 was associated with lymph node and distant metastasis, advanced clinical stage (P < 0.001), and shorter overall survival in CRCs. Cox regression analysis indicated that high tumor-TAGLN2 expression represents an independent prognostic factor. Consequently, over-expression of TAGLN2 may serve as a new biomarker for predicting progression and prognosis of CRC. (Cancer Sci 2010; 101: 523-529)

语种: 英语
所属项目编号: 2005-2010
项目资助者: Capital Medical Development Foundation of the Beijing Municipal Health Bureau, P.R. China
WOS记录号: WOS:000274156400034
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58663
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Beijing Proteome Res Ctr, State Key Lab Prote, Beijing, Peoples R China
2.Peking Univ, Peking Univ Peoples Hosp, Dept Surg Gastroenterol, Beijing 100871, Peoples R China
3.Peking Univ, Peking Univ Peoples Hosp, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Yanbin,Ye, Yingjiang,Shen, Danhua,et al. Identification of transgelin-2 as a biomarker of colorectal cancer by laser capture microdissection and quantitative proteome analysis[J]. CANCER SCIENCE,2010,101(2):523-529.
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