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学科主题: 基础医学
题名:
Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells
作者: Li, Ying-Bo1,2; Wang, Rui1,2; Wu, Hong-Li1,2; Li, Yu-Hua1,2; Zhong, Li-Jun3; Yu, He-Ming4; Li, Xuejun1,2
关键词: Ganoderma lucidum (Leyss. ex Fr.) Karst ; polysaccharides ; anti-tumor ; two dimensional gel electrophoresis ; mass spectrometry ; serum amyloid A ; adhesion
刊名: ONCOLOGY REPORTS
发表日期: 2008-09-01
DOI: 10.3892/or_00000041
卷: 20, 期:3, 页:549-556
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: ARG-GLY-ASP ; IN-VITRO ; LUNG-CARCINOMA ; PROTEIN ; CANCER ; ANGIOGENESIS ; PEPTIDE ; MATRIX ; DEGRADATION ; METASTASIS
英文摘要:

Ganoderma ludicum polysaccharides (GIPS) are the major bioactive composition of Ganoderma lucidum, a well-recognized oriental medical fungus. The published data have shown a complementary effect of GlPS in cancer therapy. The present study was designed to determine the anti-tumor efficacy of GIPS and the possible mechanism covering this effect. Murine Sarcoma 180 (S180) model was established, and GIPS administered orally for 10 days. On the 10th day, tumors were weighed to assess the inhibitory effect of GIPS and sera were collected for proteomic analysis and in vitro study. The in vivo results demonstrated that 25, 50 and 100 mg/kg GIPS inhibited S 180 growth by 32.67, 44.80 and 45.24%, respectively (P < 0.01). Proteomic study revealed marked protein changes after the process of treatment. Three significantly changed proteins were identified by ESI-Q-TOF-MS and database search indicated that they were haptoblobin, apolipoprotein A-II and serum amyloid A (SAA), respectively. Additionally, the expression change of SAA was confirmed by both Western blot and RT-PCR. The adhesion assay showed that GIPS-treated sera dramatically inhibited the adhesion ability of human prostate carcinoma (PC-3M) cells to human umbilical cord vascular endothelial cells (HUVECs), and this effect partially recovered after immunodepletion by the antibody against SAA. Collectively, these results suggest that GIPS inhibited the tumor growth and tumor cell adhesion to HUVECs via up-regulation of SAA protein expression.

语种: 英语
所属项目编号: 30171090 ; 30270528 ; 30572202 ; 30772571 ; 2004CB518902 ; B0700
项目资助者: Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China
WOS记录号: WOS:000259259200011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58688
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing 100081, Peoples R China

Recommended Citation:
Li, Ying-Bo,Wang, Rui,Wu, Hong-Li,et al. Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells[J]. ONCOLOGY REPORTS,2008,20(3):549-556.
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