IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells
Li, Ying-Bo1,2; Wang, Rui1,2; Wu, Hong-Li1,2; Li, Yu-Hua1,2; Zhong, Li-Jun3; Yu, He-Ming4; Li, Xuejun1,2
关键词Ganoderma lucidum (Leyss. ex Fr.) Karst polysaccharides anti-tumor two dimensional gel electrophoresis mass spectrometry serum amyloid A adhesion
刊名ONCOLOGY REPORTS
2008-09-01
DOI10.3892/or_00000041
20期:3页:549-556
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China ; Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China
研究领域[WOS]Oncology
关键词[WOS]ARG-GLY-ASP ; IN-VITRO ; LUNG-CARCINOMA ; PROTEIN ; CANCER ; ANGIOGENESIS ; PEPTIDE ; MATRIX ; DEGRADATION ; METASTASIS
英文摘要

Ganoderma ludicum polysaccharides (GIPS) are the major bioactive composition of Ganoderma lucidum, a well-recognized oriental medical fungus. The published data have shown a complementary effect of GlPS in cancer therapy. The present study was designed to determine the anti-tumor efficacy of GIPS and the possible mechanism covering this effect. Murine Sarcoma 180 (S180) model was established, and GIPS administered orally for 10 days. On the 10th day, tumors were weighed to assess the inhibitory effect of GIPS and sera were collected for proteomic analysis and in vitro study. The in vivo results demonstrated that 25, 50 and 100 mg/kg GIPS inhibited S 180 growth by 32.67, 44.80 and 45.24%, respectively (P < 0.01). Proteomic study revealed marked protein changes after the process of treatment. Three significantly changed proteins were identified by ESI-Q-TOF-MS and database search indicated that they were haptoblobin, apolipoprotein A-II and serum amyloid A (SAA), respectively. Additionally, the expression change of SAA was confirmed by both Western blot and RT-PCR. The adhesion assay showed that GIPS-treated sera dramatically inhibited the adhesion ability of human prostate carcinoma (PC-3M) cells to human umbilical cord vascular endothelial cells (HUVECs), and this effect partially recovered after immunodepletion by the antibody against SAA. Collectively, these results suggest that GIPS inhibited the tumor growth and tumor cell adhesion to HUVECs via up-regulation of SAA protein expression.

语种英语
所属项目编号30171090 ; 30270528 ; 30572202 ; 30772571 ; 2004CB518902 ; B0700
资助者Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China ; Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China
WOS记录号WOS:000259259200011
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58688
专题北京大学基础医学院_药理学系
作者单位1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Li, Ying-Bo,Wang, Rui,Wu, Hong-Li,et al. Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells[J]. ONCOLOGY REPORTS,2008,20(3):549-556.
APA Li, Ying-Bo.,Wang, Rui.,Wu, Hong-Li.,Li, Yu-Hua.,Zhong, Li-Jun.,...&Li, Xuejun.(2008).Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells.ONCOLOGY REPORTS,20(3),549-556.
MLA Li, Ying-Bo,et al."Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells".ONCOLOGY REPORTS 20.3(2008):549-556.
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