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学科主题临床医学
Cryoloop vitrification of rabbit oocytes
Cai, XY; Chen, GA; Lian, Y; Zheng, XY; Peng, HM
关键词cryoloop cryopreservation oocyte rabbit spindle vitrification
刊名HUMAN REPRODUCTION
2005-07-01
DOI10.1093/humrep/deh805
20期:7页:1969-1974
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]INTRACYTOPLASMIC SPERM INJECTION ; IN-VITRO FERTILIZATION ; MEIOTIC SPINDLE ; ETHYLENE-GLYCOL ; MOUSE OOCYTES ; CRYOPRESERVATION ; EMBRYOS ; PREGNANCY ; PERSPECTIVES ; ORGANIZATION
英文摘要

BACKGROUND: Vitrification is assumed to be a promising method to cryopreserve human oocytes but still needs optimization. In this study, rabbit oocytes (fertilized by ICSI) were vitrified with cryoloops, and the effect of three different cryopreservation protocols on spindle configuration and embryo quality was assessed. METHODS: Metaphase II rabbit oocytes were randomly assigned to one of four groups: (i) control; (ii) E40 [40% ethylene glycol (EG)]; (iii) ED20 [20% EG + 20% dimethylsulphoxide (DMSO)]; and (iv) ED20 + M (20% EG + 20% DMSO + vitrification machine). After warming, one part of each group was fertilized by ICSI to examine the fertilization and embryo cleavage ability, and the others were immunostained for tubulin and chromatin before visualization using confocal microscopy. RESULTS: The survival rates after warming were 79.1, 83.1 and 82.3%, respectively. In protocols E40 and ED20, the spindles were severely injured and the embryo quality not good compared with those in the ED20 + M group. CONCLUSIONS: The fastest cooling rate in combination with EG and DMSO as cryoprotectants had the fewest adverse effects on the spindle configuration of rabbit oocytes and embryo development.

语种英语
WOS记录号WOS:000230359600033
引用统计
被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58690
专题北京大学第三临床医学院
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynaecol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Cai, XY,Chen, GA,Lian, Y,et al. Cryoloop vitrification of rabbit oocytes[J]. HUMAN REPRODUCTION,2005,20(7):1969-1974.
APA Cai, XY,Chen, GA,Lian, Y,Zheng, XY,&Peng, HM.(2005).Cryoloop vitrification of rabbit oocytes.HUMAN REPRODUCTION,20(7),1969-1974.
MLA Cai, XY,et al."Cryoloop vitrification of rabbit oocytes".HUMAN REPRODUCTION 20.7(2005):1969-1974.
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