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学科主题: 临床医学
题名:
Cryoloop vitrification of rabbit oocytes
作者: Cai, XY; Chen, GA; Lian, Y; Zheng, XY; Peng, HM
关键词: cryoloop ; cryopreservation ; oocyte ; rabbit ; spindle ; vitrification
刊名: HUMAN REPRODUCTION
发表日期: 2005-07-01
DOI: 10.1093/humrep/deh805
卷: 20, 期:7, 页:1969-1974
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: INTRACYTOPLASMIC SPERM INJECTION ; IN-VITRO FERTILIZATION ; MEIOTIC SPINDLE ; ETHYLENE-GLYCOL ; MOUSE OOCYTES ; CRYOPRESERVATION ; EMBRYOS ; PREGNANCY ; PERSPECTIVES ; ORGANIZATION
英文摘要:

BACKGROUND: Vitrification is assumed to be a promising method to cryopreserve human oocytes but still needs optimization. In this study, rabbit oocytes (fertilized by ICSI) were vitrified with cryoloops, and the effect of three different cryopreservation protocols on spindle configuration and embryo quality was assessed. METHODS: Metaphase II rabbit oocytes were randomly assigned to one of four groups: (i) control; (ii) E40 [40% ethylene glycol (EG)]; (iii) ED20 [20% EG + 20% dimethylsulphoxide (DMSO)]; and (iv) ED20 + M (20% EG + 20% DMSO + vitrification machine). After warming, one part of each group was fertilized by ICSI to examine the fertilization and embryo cleavage ability, and the others were immunostained for tubulin and chromatin before visualization using confocal microscopy. RESULTS: The survival rates after warming were 79.1, 83.1 and 82.3%, respectively. In protocols E40 and ED20, the spindles were severely injured and the embryo quality not good compared with those in the ED20 + M group. CONCLUSIONS: The fastest cooling rate in combination with EG and DMSO as cryoprotectants had the fewest adverse effects on the spindle configuration of rabbit oocytes and embryo development.

语种: 英语
WOS记录号: WOS:000230359600033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58690
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Obstet & Gynaecol, Beijing 100083, Peoples R China

Recommended Citation:
Cai, XY,Chen, GA,Lian, Y,et al. Cryoloop vitrification of rabbit oocytes[J]. HUMAN REPRODUCTION,2005,20(7):1969-1974.
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