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学科主题: 临床医学
题名:
Reduction in VEGF Protein and Phosphorylated Nephrin Associated with Proteinuria in Adriamycin Nephropathy Rats
作者: Fan, Qingfeng1; Xing, Yan2; Ding, Jie1; Guan, Na1
关键词: Vascular endothelial growth factor ; Nephrin phosphorylation ; Prednisone ; Lisinopril ; Proteinuria
刊名: NEPHRON EXPERIMENTAL NEPHROLOGY
发表日期: 2009
DOI: 10.1159/000209209
卷: 111, 期:4, 页:E92-E102
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; PUROMYCIN AMINONUCLEOSIDE NEPHROSIS ; PODOCYTE-ASSOCIATED MOLECULES ; DIABETIC-NEPHROPATHY ; SLIT DIAPHRAGM ; EXPRESSION ; COMPONENTS ; KIDNEY ; CD2AP
英文摘要:

Background/Aims: The relationship between vascular endothelial growth factor (VEGF) and the phosphorylated critical podocyte slit diaphragm molecule nephrin is not fully clarified. This study investigated the dynamic changes in VEGF expression and nephrin phosphorylation, and the effects of the antiproteinuric drugs prednisone and lisinopril on them in Adriamycin nephropathy rats. Methods: Renal tissues from Adriamycin rats were collected at days 3, 7, 14, and 28. Distribution and expression of VEGF was revealed by immunohistochemistry, real-time PCR and Western blot. Phosphorylated nephrin was evaluated by immunoprecipitation. Results: A discontinuous redistribution of VEGF was displayed at day 3, followed by significant protein reduction at day 7 with persistent downregulation to day 28. Phosphorylated nephrin decreased evidently at day 14 and persisted to day 28. The reduction in VEGF and phosphorylated nephrin was not a result of podocyte loss. The intervention of prednisone and lisinopril evidently reduced proteinuria, effectively attenuated the severe lesions of podocyte foot processes, and restored the reduction in VEGF and nephrin phosphorylation. At day 28, the reduction in VEGF and phosphorylated nephrin was negatively correlated with proteinuria, whereas the phosphorylated nephrin was positively correlated with VEGF protein from day 7 to day 28. Conclusion: The reduction in VEGF protein and nephrin phosphorylation was possibly involved in the proteinuria in Adriamycin rats, and there might be some relationship between VEGF and nephrin phosphorylation. The antiproteinuric effects of lisinopril and prednisone were achieved at least partially by restoring VEGF protein and nephrin phosphorylation. Copyright (C) 2009 S. Karger AG, Basel

语种: 英语
所属项目编号: 30170992 ; 30672259
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000265122800003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58696
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Fan, Qingfeng,Xing, Yan,Ding, Jie,et al. Reduction in VEGF Protein and Phosphorylated Nephrin Associated with Proteinuria in Adriamycin Nephropathy Rats[J]. NEPHRON EXPERIMENTAL NEPHROLOGY,2009,111(4):E92-E102.
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