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学科主题: 临床医学
题名:
Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements
作者: Lin, Guiting1; Alwaal, Amjad1; Sun, Fionna1; Zhang, Haiyang1; Li, Huixi1,2,3; Wang, Lin1,2,3; Wang, Guifang1; Ning, Hongxiu1; Banie, Lia1; Lin, Ching-Shwun1; Lue, Tom F.1
关键词: urethra ; muscle, smooth ; elastic tissue ; estrogens ; transforming growth factor beta
刊名: JOURNAL OF UROLOGY
发表日期: 2015-06-01
DOI: 10.1016/j.juro.2014.12.085
卷: 193, 期:6, 页:2131-2137
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: STRESS URINARY-INCONTINENCE ; PELVIC ORGAN PROLAPSE ; GROWTH-FACTOR-BETA ; CROSS-TALK ; TISSUE ; WOMEN ; TRANSCRIPTION ; DYSFUNCTION ; SYSTEM ; CANCER
英文摘要:

Purpose: We investigated the effect and mechanism of estrogen on elastogenesis in urethral smooth muscle cells in vitro.

Materials and Methods: Urethral smooth muscle cells were isolated from normal adult female rats. For elastogenesis assay cells were treated with TGF-beta 1, the potent TGF-beta 1 receptor inhibitor SB431542 and estrogen for 2 weeks. Real-time polymerase chain reaction was performed to assay gene expression during this process. Activity of the TGF-beta 1 responsive elements CAGA(12)-Luc and GCCG(12)-Luc were also assayed. Estrogen receptor and Smad2/3 interaction was evaluated by immunoprecipitation and Western blot.

Results: TGF-beta 1 induced elastogenesis in rat urethral smooth muscle cells. This effect was partially blocked by estrogen and completely abrogated by SB431542. SB431542 completely inhibited activation of the Smad2/3 response element CAGA(12)-Luc and estrogen significantly inhibited activation. The Smad1/4 response element GCCG(12)-Luc was not affected by SB431542 treatment but estrogen partially inhibited the activation of GCCG(12)-Luc induced by TGF-beta 1. Estrogen receptor bound to Smad 2 and 3 in vitro.

Conclusions: Estrogen attenuated TGF-beta 1 induced elastogenesis via binding of its activated receptor to Smad2/3 to inhibit the TGF-beta 1 response element in rat urethral smooth muscle cells.

语种: 英语
所属项目编号: DK069655
项目资助者: National Institutes of Health
WOS记录号: WOS:000354196700101
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58697
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
3.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA

Recommended Citation:
Lin, Guiting,Alwaal, Amjad,Sun, Fionna,et al. Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements[J]. JOURNAL OF UROLOGY,2015,193(6):2131-2137.
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