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学科主题临床医学
Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements
Lin, Guiting1; Alwaal, Amjad1; Sun, Fionna1; Zhang, Haiyang1; Li, Huixi1,2,3; Wang, Lin1,2,3; Wang, Guifang1; Ning, Hongxiu1; Banie, Lia1; Lin, Ching-Shwun1; Lue, Tom F.1
关键词urethra muscle, smooth elastic tissue estrogens transforming growth factor beta
刊名JOURNAL OF UROLOGY
2015-06-01
DOI10.1016/j.juro.2014.12.085
193期:6页:2131-2137
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]STRESS URINARY-INCONTINENCE ; PELVIC ORGAN PROLAPSE ; GROWTH-FACTOR-BETA ; CROSS-TALK ; TISSUE ; WOMEN ; TRANSCRIPTION ; DYSFUNCTION ; SYSTEM ; CANCER
英文摘要

Purpose: We investigated the effect and mechanism of estrogen on elastogenesis in urethral smooth muscle cells in vitro.

Materials and Methods: Urethral smooth muscle cells were isolated from normal adult female rats. For elastogenesis assay cells were treated with TGF-beta 1, the potent TGF-beta 1 receptor inhibitor SB431542 and estrogen for 2 weeks. Real-time polymerase chain reaction was performed to assay gene expression during this process. Activity of the TGF-beta 1 responsive elements CAGA(12)-Luc and GCCG(12)-Luc were also assayed. Estrogen receptor and Smad2/3 interaction was evaluated by immunoprecipitation and Western blot.

Results: TGF-beta 1 induced elastogenesis in rat urethral smooth muscle cells. This effect was partially blocked by estrogen and completely abrogated by SB431542. SB431542 completely inhibited activation of the Smad2/3 response element CAGA(12)-Luc and estrogen significantly inhibited activation. The Smad1/4 response element GCCG(12)-Luc was not affected by SB431542 treatment but estrogen partially inhibited the activation of GCCG(12)-Luc induced by TGF-beta 1. Estrogen receptor bound to Smad 2 and 3 in vitro.

Conclusions: Estrogen attenuated TGF-beta 1 induced elastogenesis via binding of its activated receptor to Smad2/3 to inhibit the TGF-beta 1 response element in rat urethral smooth muscle cells.

语种英语
WOS记录号WOS:000354196700101
项目编号DK069655
资助机构National Institutes of Health
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58697
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
3.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
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Lin, Guiting,Alwaal, Amjad,Sun, Fionna,et al. Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements[J]. JOURNAL OF UROLOGY,2015,193(6):2131-2137.
APA Lin, Guiting.,Alwaal, Amjad.,Sun, Fionna.,Zhang, Haiyang.,Li, Huixi.,...&Lue, Tom F..(2015).Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements.JOURNAL OF UROLOGY,193(6),2131-2137.
MLA Lin, Guiting,et al."Estrogen Attenuates TGF-beta 1 Induced Elastogenesis in Rat Urethral Smooth Muscle Cells by Inhibiting Smad Response Elements".JOURNAL OF UROLOGY 193.6(2015):2131-2137.
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