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学科主题: 临床医学
题名:
bcl 10 gene mutation in hepatocellular carcinoma
作者: Cheng, JH; Leng, XS; Cai, SL; Cao, ZX; Cao, G; Peng, JR; Wang, SW; Du, RY
关键词: bcl 10 gene ; liver cancer ; mutation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-07-01
卷: 114, 期:7, 页:747-751
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CARD
英文摘要:

Objective To detect the mutation frequency of the bcl 10 gene in the early and advanced stages of hepatocellular carcinoma (HCC).

Methods Genome DNA samples were extracted from 46 cases of fresh HCC tumor tissues and their nontumor adjacent tissues. Polymerase chain reaction-single strand conformation polymorphism method was used to detect point mutations of the three exons of the bcl 10 gene. For each individual exon, six random samples from those showing abnormal DNA bands were sequenced to verify those mutations. The relationship between serum alpha-fetoprotein (AFP) level and bcl 10 mutation, between the tumor size and bcl 10 mutation was also analyzed.

Results Among the 46 samples, 26 cases (56.5%) were found to have mutations in exon 1, 5 out of the 6 cases were shown to have 5744 C -->G mutation by sequencing; 25 cases (54.3%) were found to have mutations in exon 2, 4 out of the 6 cases were shown to have 11 311 T deletion mutation by sequencing. Twenty-one cases (45.7%) were found to have mutations in exon 3, all of the 6 cases selected for sequencing were shown to have 14 116 C -->T mutation. Statistical analysis showed that neither serum alpha-fetoprotein level nor the size of hepatocellular carcinoma has a significant relationship with bcl 10 mutation.

Conclusion The bcl 10 gene has a high mutation frequency in liver cancer.

语种: 英语
WOS记录号: WOS:000170838300019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58703
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing Univ, Peoples Hosp, Dept Surg, Beijing 100044, Peoples R China
2.Capital Univ Med Sci, Anzhen Hosp, Dept Gen Surg, Beijing 100029, Peoples R China

Recommended Citation:
Cheng, JH,Leng, XS,Cai, SL,et al. bcl 10 gene mutation in hepatocellular carcinoma[J]. CHINESE MEDICAL JOURNAL,2001,114(7):747-751.
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