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Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection
Li, Min; Zhao, Hong-Cui; Li, Rong; Yu, Yang; Qiao, Jie
刊名PLOS ONE
2014-07-24
DOI10.1371/journal.pone.0103347
9期:7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program ; Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MEIOTIC SPINDLE ; BOVINE OOCYTES ; HUMAN EMBRYOS ; OVARIAN HYPERSTIMULATION ; BLASTOMERE BIOPSY ; PRIMITIVE STREAK ; ICSI OUTCOMES ; MATURATION ; FERTILIZATION ; ANEUPLOIDY
英文摘要

Implantation failure and early pregnancy loss have been reported to be closely related to the quality of mammalian oocytes; however, the pregnant outcome of embryos from in-vitro matured (IVM) oocytes remains unknown. In this study we examined spindle assembly and chromosome segregation during differentiation, and the duration of IVM of mouse oocytes. The resulting implantation and pregnancy outcomes were analyzed to clarify the relationship between the spindle and chromosomes of IVM oocytes and implantation and early pregnancy. Cumulus-enclosed germinal vesicle oocytes were collected and randomly cultured in IVM medium with different IVM durations. One part of IVM oocytes were analyzed the spindle and chromosome morphology by immunofluorescence method, and the other part of them were fertilized by intracytoplasmic sperm injection. The resulting embryos were transferred into pseudo-pregnant female mice, and the post-implantation and full term development was observed. The chromosome aberrations and incorrect spindle assembly seems not affect the early development and blastocyst cell number derived from IVM oocytes, however the development potential of the resulting embryos after implantation were significant decreased with the ratio increasing of chromosome aberrations and incorrect spindle assembly. Accordingly, the full-term development was also decreased. In conclusion, the present study showed the spindle assembly of in vitro-matured oocytes was one of the most important factors that affected the implantation and ongoing pregnancy rates of IVM oocytes, and the improvement by an appropriate duration of maturation in vitro will enhance the post-implantation development potential of the resulting embryos, and decrease implantation failure and early pregnancy loss.

语种英语
所属项目编号2014CB943203 ; 31371521
资助者Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program ; Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program
WOS记录号WOS:000341354800088
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58705
专题北京大学第三临床医学院_生殖医学中心
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li, Min,Zhao, Hong-Cui,Li, Rong,et al. Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection[J]. PLOS ONE,2014,9(7).
APA Li, Min,Zhao, Hong-Cui,Li, Rong,Yu, Yang,&Qiao, Jie.(2014).Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection.PLOS ONE,9(7).
MLA Li, Min,et al."Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection".PLOS ONE 9.7(2014).
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