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学科主题临床医学
Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo
Liu, F; Lu, DP
关键词placenta-eluted gamma globulin T-cell bone marrow transplantation graft-versus-host disease
刊名INTERNATIONAL JOURNAL OF HEMATOLOGY
2005-08-01
DOI10.1532/IJH97.A10506
82期:2页:162-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
研究领域[WOS]Hematology
关键词[WOS]RHEUMATOID-ARTHRITIS ; T-CELLS ; BLOCKING ANTIBODIES ; CD69 EXPRESSION ; ACTIVATION ; DISEASE ; IMMUNOGLOBULINS ; ALLOANTIBODIES ; COSTIMULATION ; KINETICS
英文摘要

In the 1980s, attempts were made to use placenta-eluted gamma globulins (PEGG) in patients with graft-versus-host disease (GVHD) after bone marrow transplantation (BMT). Because production of PEGG had been discontinued for many years, we aimed to reestablish a method of production and further explore the mechanisms of the effect of these globulins on GVHD. PEGG were prepared by elution at acid pH from extensively washed human placenta followed by precipitation with saturated ammonium sulfate and absorption on a protein A Sepharose column. In vitro study showed PEGG significantly inhibited both the proliferative response of T-cells to phytohemagglutinin (PHA) and the mixed lymphocyte reaction (MLR). Results of flow cytometric analysis indicated that PEGG down-regulated the expression of CD25 and CD69 on T-cells stimulated by PHA. Cytokine quantification in NILR supernatant showed that PEGG decreased secretion of interferon gamma (IFN-gamma) but increased production of interleukin 4. In a murine GVHD model, we investigated the preventive effect of PEGG on lethal GVHD in irradiated recipients of allogeneic bone marrow cells and spleen cell transplants by in vivo administration. Compared with controls, recipients treated with PEGG had a markedly increased survival rate with less histopathological evidence of GVHD. These results suggest that PEGG may be a potent therapeutic agent for GVHD.

语种英语
WOS记录号WOS:000231530000015
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58713
专题北京大学第二临床医学院_血液科
作者单位Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
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Liu, F,Lu, DP. Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2005,82(2):162-168.
APA Liu, F,&Lu, DP.(2005).Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo.INTERNATIONAL JOURNAL OF HEMATOLOGY,82(2),162-168.
MLA Liu, F,et al."Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo".INTERNATIONAL JOURNAL OF HEMATOLOGY 82.2(2005):162-168.
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