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学科主题: 临床医学
题名:
Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo
作者: Liu, F; Lu, DP
关键词: placenta-eluted gamma globulin ; T-cell ; bone marrow transplantation ; graft-versus-host disease
刊名: INTERNATIONAL JOURNAL OF HEMATOLOGY
发表日期: 2005-08-01
DOI: 10.1532/IJH97.A10506
卷: 82, 期:2, 页:162-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: RHEUMATOID-ARTHRITIS ; T-CELLS ; BLOCKING ANTIBODIES ; CD69 EXPRESSION ; ACTIVATION ; DISEASE ; IMMUNOGLOBULINS ; ALLOANTIBODIES ; COSTIMULATION ; KINETICS
英文摘要:

In the 1980s, attempts were made to use placenta-eluted gamma globulins (PEGG) in patients with graft-versus-host disease (GVHD) after bone marrow transplantation (BMT). Because production of PEGG had been discontinued for many years, we aimed to reestablish a method of production and further explore the mechanisms of the effect of these globulins on GVHD. PEGG were prepared by elution at acid pH from extensively washed human placenta followed by precipitation with saturated ammonium sulfate and absorption on a protein A Sepharose column. In vitro study showed PEGG significantly inhibited both the proliferative response of T-cells to phytohemagglutinin (PHA) and the mixed lymphocyte reaction (MLR). Results of flow cytometric analysis indicated that PEGG down-regulated the expression of CD25 and CD69 on T-cells stimulated by PHA. Cytokine quantification in NILR supernatant showed that PEGG decreased secretion of interferon gamma (IFN-gamma) but increased production of interleukin 4. In a murine GVHD model, we investigated the preventive effect of PEGG on lethal GVHD in irradiated recipients of allogeneic bone marrow cells and spleen cell transplants by in vivo administration. Compared with controls, recipients treated with PEGG had a markedly increased survival rate with less histopathological evidence of GVHD. These results suggest that PEGG may be a potent therapeutic agent for GVHD.

语种: 英语
WOS记录号: WOS:000231530000015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58713
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China

Recommended Citation:
Liu, F,Lu, DP. Purification of placenta-eluted gamma globulins and their strong effect against graft-versus-host reactions in vitro and in vivo[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2005,82(2):162-168.
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