北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病理学系  > 期刊论文
学科主题: 基础医学
题名:
Tyrphostin AG1478 suppresses proliferation and invasion of human breast cancer cells
作者: Zhang, Yun-Gang1,2; Du, Qiang1; Fang, Wei-Gang1; Jin, Mu-Lan2; Tian, Xin-Xia1
关键词: breast cancer ; epidermal growth factor receptor ; Tyrphostin AG1478
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2008-09-01
DOI: 10.3892/ijo_00000045
卷: 33, 期:3, 页:595-602
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GROWTH-FACTOR-RECEPTOR ; TYROSINE KINASE INHIBITOR ; CARCINOMA-CELLS ; TELOMERASE ACTIVITY ; PANCREATIC-CANCER ; EPITHELIAL-CELLS ; DRUG-RESISTANCE ; LUNG-CANCER ; PHASE-II ; EXPRESSION
英文摘要:

Inhibition of epidermal growth factor receptor (EGFR) signaling is a promising treatment strategy for malignant tumors. In this study, we evaluated the effectiveness of Tyrphostin AG1478, a potent and specific inhibitor of EGFR tyrosine kinase, on the growth, apoptosis and invasion of breast cancer cells. Western blotting demonstrated that AG1478 inhibited the phosphorylation of EGFR, ERK1/2 and AKT in a dose-dependent manner. Three proliferation analyses, MTT, cell counting, and clone formation assay, consistently showed that AG1478 significantly inhibited cell proliferation in a dose-dependent manner. FACS analysis demonstrated that AG1478 promoted cell apoptosis. In addition, TRAP assay exhibited that AG1478 significantly suppressed telomerase activity of tumor cells, which was parallel with growth inhibition. Semi-qantitative RT-PCR revealed that the suppression of telomerase activity was correlated with the decreased expression of human telomerase catalytic subunit (hTERT) mRNA, the rate-limiting determinant of its enzyme activity. These data suggest that AG1478 suppressed cellular growth by inhibiting cellular proliferation, inducing apoptosis and inhibiting telomerase activity. Furthermore, we also examined the effects of AG1478 on cellular invasion. Boyden chamber invasion assay showed that AG1478 significantly inhibited cell invasion in a dose-dependent manner. Western blotting revealed that AG1478 could down-regulate the expression of MMP-9, which may be one of the mechanisms by which AG1478 suppressed cellular invasion. In conclusion, this study demonstrated that Tyrphostin AG1478 effectively inhibited the proliferation and invasion of breast cancer cells. Tyrphostin AG1478 may be a potential EGFR-targeted therapeutic agent for breast cancer.

语种: 英语
所属项目编号: 30670805 ; 30770829
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000259519500022
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58723
Appears in Collections:基础医学院_病理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Tyrphostin AG1478 suppresses proliferation and invasion of human breast cancer cells.pdf(293KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
2.Capital Med Univ, Beijing Chao Yang Hosp, Dept Pathol, Beijing 100020, Peoples R China

Recommended Citation:
Zhang, Yun-Gang,Du, Qiang,Fang, Wei-Gang,et al. Tyrphostin AG1478 suppresses proliferation and invasion of human breast cancer cells[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2008,33(3):595-602.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yun-Gang]'s Articles
[Du, Qiang]'s Articles
[Fang, Wei-Gang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yun-Gang]‘s Articles
[Du, Qiang]‘s Articles
[Fang, Wei-Gang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace