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学科主题临床医学
Superoxide anion, the main species of ROS in the development of ARDS induced by oleic acid
Liu, HL; Zhang, DL; Zhao, BL; Zhao, JY
关键词reactive oxygen species acute respiratory distress syndrome electron spin resonance ultra-weak luminescence superoxide dismutase
刊名FREE RADICAL RESEARCH
2004-12-01
DOI10.1080/10715760400006940
38期:12页:1281-1287
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]RESPIRATORY-DISTRESS-SYNDROME ; ACUTE LUNG INJURY ; OXIDATIVE STRESS ; ANTIOXIDANTS ; HYDROXYL ; RADICALS ; DISEASE
英文摘要

It is believed that reactive oxygen species (ROS) play a very important role in the pathogenesis of acute respiratory distress syndrome (ARDS), but the mechanism has not been so clear, owing to the absence of direct measurable (experimental) data. In majority of the medical studies on free radicals, the analysis of ROS has generally been done by the way of measuring their secondary and breakdown products. In our study, we used electron spin resonance (ESR), a sensitive and accurate technique to detect ROS directly and also used some other sensitive techniques including ultra-weak luminescence and chemical luminescence to identify the species and relative amount of ROS. Furthermore, superoxide dismutase (SOD) was pre-administrated in ARDS rats to verify the results from the above studies and explore the possibility of the clinical application of SOD in ARDS. The spectra of ESR showed that the concentration of ROS increased at 10 min and reached a summit at 30 min after injection of oleic acid (OA), then dropped gradually. The scavenging effects of different scavenging agents on ROS by the analysis of ultra-weak luminescence proved that superoxide anion was the main species of ROS in the development of OA-induced ARDS. Moreover, the results of quantified measure of superoxide anion by chemical luminescence also showed the accordant tendency exhibited in ESR measurement. The pre-treatment of SOD might distinctly inhibit the production of superoxide anion, obviously improve the blood gas status, lung wet/dry ratio and lung/body ratio in ARDS rats. It is suggested that ROS may play a key role in the initiation phase of ARDS, while superoxide anion may be a leading actor in this process and SOD could effectively protect rats from ARDS. These results may provide helpful information for the treatment and prevention of ARDS.

语种英语
WOS记录号WOS:000226032000003
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58725
专题北京大学第三临床医学院_职业病科
作者单位1.Peking Univ, Hosp 3, Res Ctr Occupat Med, Beijing 100083, Peoples R China
2.Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Liu, HL,Zhang, DL,Zhao, BL,et al. Superoxide anion, the main species of ROS in the development of ARDS induced by oleic acid[J]. FREE RADICAL RESEARCH,2004,38(12):1281-1287.
APA Liu, HL,Zhang, DL,Zhao, BL,&Zhao, JY.(2004).Superoxide anion, the main species of ROS in the development of ARDS induced by oleic acid.FREE RADICAL RESEARCH,38(12),1281-1287.
MLA Liu, HL,et al."Superoxide anion, the main species of ROS in the development of ARDS induced by oleic acid".FREE RADICAL RESEARCH 38.12(2004):1281-1287.
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