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Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector
Yue, Xinye1; Zhang, Wendi1; Xing, Jinfeng1; Zhang, Biao1; Deng, Liandong1; Guo, Shutao1; Yang, Jun3; Zhang, Qiang4; Dong, Anjie1,2
刊名SOFT MATTER
2012
DOI10.1039/c2sm07068e
8期:7页:2252-2260
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Physical ; Materials Science, Multidisciplinary ; Physics, Multidisciplinary ; Polymer Science
研究领域[WOS]Chemistry ; Materials Science ; Physics ; Polymer Science
关键词[WOS]CORE-SHELL NANOPARTICLES ; BLOCK-COPOLYMER ; MICELLAR NANOPARTICLES ; EFFICIENT GENE ; DRUG-DELIVERY ; TRANSFECTION ; CARRIERS ; THERAPY ; SYSTEMS
英文摘要

In this study, well-defined amphiphilic cationic triblock copolymers with different lengths of polycationic chain, methoxy poly(ethylene glycol)-b-poly(D, L-lactide)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PDLLA-b-PDMA), were synthesized and evaluated as carriers for gene delivery. The prepared copolymers can self-assemble into spherical core-shell nanoparticles (NPs). The NPs have a very low critical micelle concentration (CMC) value with only 0.025 mg mL(-1). Both copolymers can completely condense the pDNA into spherical complexes when the N/P ratio is equal to or above 3. Bovine serum albumin challenging and DNase I protection assay results demonstrate the mPEG-b-PDLLA-b-PDMA NPs can effectively protect the DNA against protein and nucleases. MTT assay results indicate that mPEG-b-PDLLA-b-PDMA NP/pDNA complexes exhibit obviously lower cytotoxicity compared with commercial gene transfection reagent Lipofectamine 2000/pDNA complexes. Subsequently, in vitro gene transfection studies in HeLa cells without serum show that mPEG(113)-b-PDLLA(10)-b-PDMA(120) NP/pDNA complexes exhibit higher transfection efficiency than Lipofectamine 2000. Although the transfection efficiency is lower than that in the absence of serum, mPEG(113)-b-PDLLA(10)-b-PDMA(120) NPs still display equivalent gene transfection efficiency compared to Lipofectamine 2000 when N/P ratio is above 15 in DMEM with 10% serum.

语种英语
WOS记录号WOS:000299477300022
项目编号2009CB930300 ; 31100722 ; 30772007 ; 20090032120013
资助机构973 Program ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58749
专题北京大学药学院
作者单位1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
2.Tianjin Univ, Sch Mat Sci & Technol, Tianjin 300072, Peoples R China
3.Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Bioact Mat, Tianjin 300071, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yue, Xinye,Zhang, Wendi,Xing, Jinfeng,et al. Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector[J]. SOFT MATTER,2012,8(7):2252-2260.
APA Yue, Xinye.,Zhang, Wendi.,Xing, Jinfeng.,Zhang, Biao.,Deng, Liandong.,...&Dong, Anjie.(2012).Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector.SOFT MATTER,8(7),2252-2260.
MLA Yue, Xinye,et al."Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector".SOFT MATTER 8.7(2012):2252-2260.
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