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学科主题: 药学
题名:
Determination of homoisoflavonoids in Ophiopogon japonicus by RP-HPLC
作者: Ye, G; Ye, M; Guo, D; Huang, CG
关键词: column liquid chromatography ; quality control ; Ophiopogon japonicus ; homoisoflavonoids
刊名: CHROMATOGRAPHIA
发表日期: 2005-02-01
DOI: 10.1365/s10337-004-0486-3
卷: 61, 期:3-4, 页:121-125
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; SUBTERRANEAN PART ; KER-GAWLER ; CONSTITUENTS ; TUBER ; CONGENERS
英文摘要:

The homoisoflavonoid content of 14 samples of Ophiopogon japonicus collected from different parts of China have been investigated. Three principal homoisoflavonoids, methylophiopog-onanone A, methylophiopogonanone B, and 6-aldehydo-isoophiopogonanone A, were analyzed simultaneously by reversed-phase high-performance liquid chromatography with a binary mixture of acetonitrile and 0.3% aqueous acetic acid as mobile phase. The recovery of the method was 98.5-102.6% and good linearity (r > 0.9990) was obtained for all the homoisoflavonoids over a relatively wide concentration range. The results indicated that the homoisoflavonoid content of Ophiopogon japonicus varied significantly from species to species, and from locality to locality. The quality and identity of Ophiopogon japonicus from the herb market can be determined from the homoisoflavonoid content.

语种: 英语
WOS记录号: WOS:000227123900004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58759
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Ye, G,Ye, M,Guo, D,et al. Determination of homoisoflavonoids in Ophiopogon japonicus by RP-HPLC[J]. CHROMATOGRAPHIA,2005,61(3-4):121 !E
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