|Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China|
|Bao, Yi; Tian, Di; Zheng, Ying-Ying; Xi, Hong-Li; Liu, Dan; Yu, Min; Xu, Xiao-Yuan|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||TREATMENT-NAIVE INDIVIDUALS ; ANTIRETROVIRAL THERAPY ; CROSS-RESISTANCE ; INFECTED INDIVIDUALS ; ENTRY INHIBITORS ; PREVALENCE ; SUBTYPE ; VIRUS ; TRANSMISSION ; REGIMENS|
Background: Natural drug resistance is a major cause of antiviral treatment failure. The characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province remain unclear.
Methods: One hundred and fifty HIV-1-positive plasma samples were collected. Plasma viral RNA was extracted for pol gene amplification and sequencing. The sequencing results were submitted to the HIV-1 drug resistance database for drug-resistance analysis.
Results: The rates of natural drug resistance and resistance-associated mutations were 17.7% (19/107) and 40.2% (43/107), respectively. The rates of PI major, PI minor, NRTI, and NNRTI mutations were: 0, 30.8% (33/107), 10.3% (11/107), and 18.7% (20/107), respectively. Nine cases (8.4%) had both NRTI and NNRTI resistance-associated mutations. Seven cases (6.5%) had PI minor, NRTI and NNRTI resistance-associated mutations. NNRTI resistance was the most serious, followed by NRTI resistance and PI resistance. Polymorphism mutation sites with mutation rates in the protease region higher than 60.0% were: L63A/P/S/T 89.7%, V77I 82.2%, I72E/M/K/T/V 80.4%, I93L 75.7%, and E35D 72.9%. Polymorphism mutation sites with mutation rates in the RT region higher than 60.0% were: I135A/L/M/R/T/V 93.5%, T200A/E/I/P/V 89.7%, Q278E/K/N/T 88.8%, S162C/Y 82.2%, and K277R/S 66.4%. The distribution of 107 gene sequences was scattered, with some drug-resistant strains grouped in the same cluster.
Conclusion: The natural drug resistance mutation rate of HIV-1 in former paid blood donors in Henan Province was 17.7%, with NNRTI resistance the most serious. The distribution of drug-resistant strains was scattered, with some correlations found in certain resistance loci.
|项目编号||30771906 ; 2008ZX10002-013 ; 20090001110081|
|资助机构||National Natural Science Foundation of China ; 11th Five-Year Plan of the National Major Project for Infectious Diseases Control ; Ph.D. Programs Foundation of Ministry of Education of China|
|作者单位||Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100871, Peoples R China|
|Bao, Yi,Tian, Di,Zheng, Ying-Ying,et al. Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China[J]. PLOS ONE,2014,9(2).|
|APA||Bao, Yi.,Tian, Di.,Zheng, Ying-Ying.,Xi, Hong-Li.,Liu, Dan.,...&Xu, Xiao-Yuan.(2014).Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China.PLOS ONE,9(2).|
|MLA||Bao, Yi,et al."Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China".PLOS ONE 9.2(2014).|