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学科主题: 临床医学
题名:
Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China
作者: Bao, Yi; Tian, Di; Zheng, Ying-Ying; Xi, Hong-Li; Liu, Dan; Yu, Min; Xu, Xiao-Yuan
刊名: PLOS ONE
发表日期: 2014-02-19
DOI: 10.1371/journal.pone.0089291
卷: 9, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: TREATMENT-NAIVE INDIVIDUALS ; ANTIRETROVIRAL THERAPY ; CROSS-RESISTANCE ; INFECTED INDIVIDUALS ; ENTRY INHIBITORS ; PREVALENCE ; SUBTYPE ; VIRUS ; TRANSMISSION ; REGIMENS
英文摘要:

Background: Natural drug resistance is a major cause of antiviral treatment failure. The characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province remain unclear.

Methods: One hundred and fifty HIV-1-positive plasma samples were collected. Plasma viral RNA was extracted for pol gene amplification and sequencing. The sequencing results were submitted to the HIV-1 drug resistance database for drug-resistance analysis.

Results: The rates of natural drug resistance and resistance-associated mutations were 17.7% (19/107) and 40.2% (43/107), respectively. The rates of PI major, PI minor, NRTI, and NNRTI mutations were: 0, 30.8% (33/107), 10.3% (11/107), and 18.7% (20/107), respectively. Nine cases (8.4%) had both NRTI and NNRTI resistance-associated mutations. Seven cases (6.5%) had PI minor, NRTI and NNRTI resistance-associated mutations. NNRTI resistance was the most serious, followed by NRTI resistance and PI resistance. Polymorphism mutation sites with mutation rates in the protease region higher than 60.0% were: L63A/P/S/T 89.7%, V77I 82.2%, I72E/M/K/T/V 80.4%, I93L 75.7%, and E35D 72.9%. Polymorphism mutation sites with mutation rates in the RT region higher than 60.0% were: I135A/L/M/R/T/V 93.5%, T200A/E/I/P/V 89.7%, Q278E/K/N/T 88.8%, S162C/Y 82.2%, and K277R/S 66.4%. The distribution of 107 gene sequences was scattered, with some drug-resistant strains grouped in the same cluster.

Conclusion: The natural drug resistance mutation rate of HIV-1 in former paid blood donors in Henan Province was 17.7%, with NNRTI resistance the most serious. The distribution of drug-resistant strains was scattered, with some correlations found in certain resistance loci.

语种: 英语
所属项目编号: 30771906 ; 2008ZX10002-013 ; 20090001110081
项目资助者: National Natural Science Foundation of China ; 11th Five-Year Plan of the National Major Project for Infectious Diseases Control ; Ph.D. Programs Foundation of Ministry of Education of China
WOS记录号: WOS:000331711900122
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58760
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100871, Peoples R China

Recommended Citation:
Bao, Yi,Tian, Di,Zheng, Ying-Ying,et al. Characteristics of HIV-1 Natural Drug Resistance-Associated Mutations in Former Paid Blood Donors in Henan Province, China[J]. PLOS ONE,2014,9(2).
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