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学科主题: 基础医学
题名:
The role of calcium ions in the interactions of PrP106-126 amide with model membranes
作者: Zhong, Jian1,2; Yang, Chunhui1,2,3; Zheng, Wenfu1,2; Huang, Lixin1,2; Hong, Yuankai1,2; Wang, Lijun1,2; Sha, Yinlin1,2
关键词: Ca2+ ; PrP106-126 amide ; Poration ; Membrane disintegration ; Atomic force microscopy
刊名: COLLOIDS AND SURFACES B-BIOINTERFACES
发表日期: 2010-05-01
DOI: 10.1016/j.colsurfb.2010.01.001
卷: 77, 期:1, 页:40-46
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
研究领域[WOS]: Biophysics ; Chemistry ; Materials Science
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; SUPPORTED LIPID-BILAYERS ; PRION PROTEIN-FRAGMENT ; SURFACE-PLASMON RESONANCE ; ANTIMICROBIAL PEPTIDES ; ELECTROLYTE-SOLUTIONS ; ALKALINE-PHOSPHATASE ; MOLECULAR-MECHANISM ; HYDRATION FORCES ; CELLS
英文摘要:

In this work, we investigated the interactions of PrP106-126 amide with 1-palmitoyl-2-oleoyl-3-phosphocholine (POPC) and POPC/bovine brain sphingomyelin (BSM) membranes in the presence of calcium ions by in situ time-lapse atomic force microscopy (AFM) and circular dichroism (CD). The CD results show that Ca2+ has no obvious effects on the random coil conformation of PrP106-126 amide. The tapping mode AFM results demonstrate that electrostatic interaction decreases the measured heights of supported lipid bilayers (SLBs) in HBS-Ca2+ solution. Electrostatic interaction analysis also can be used to determine the applied force in liquid tapping mode AFM. The interactions of PrP106-126 amide with membranes by AFM demonstrate the following: (i)Ca2+ inhibits the interaction of PrP106-126 amide with POPC lipid and (ii) the co-interaction between Ca2+ and BSM increases the poration ability of PrP106-126 amide. These results imply that the main role of Ca2+ in the interactions of PrP106-126 amide with membranes is changing the surface properties of the membranes. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 20273002 ; 20103001
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000276272100006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58761
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biophys, Singlemol & Nanobiol Lab, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China

Recommended Citation:
Zhong, Jian,Yang, Chunhui,Zheng, Wenfu,et al. The role of calcium ions in the interactions of PrP106-126 amide with model membranes[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2010,77(1):40-46.
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