IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题临床医学
Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene
Qi, YF; Bu, DF; Di Niu, D; Shi, YR; Wang, SH; Pang, YZ; Tang, CS; Du, JBA
关键词proadrenomedullin N-terminal 20 peptide (PAMP) adrenomedullin (ADM) adrenotensin (ADT) adrenomedullin gene
刊名PEPTIDES
2002-06-01
23期:6页:1141-1147
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]VASCULAR SMOOTH-MUSCLE ; COMPETITIVE PCR ; CELLS ; 20-PEPTIDE ; PROTEIN
英文摘要

Primary culture of vascular smooth muscle cells (VSMC) from rat aorta was used for the study of the effect of different peptides derived from proadrenomedullin on the expression of adrenomedullin (ADM) gene. ADM and preproADM(22-41) (PAMP) secreted by VSMC were measured by radioimmunoassay, and ADM mRNA in VSMC was determined by quantitative RT-PCR. After the incubation of VSMC in 10(-7) M ADM for 24 h, PAMP in the medium and ADM mRNA in the VSMC were decreased by 34 and 41.3%, respectively, and cAMP concentration in the VSMC was increased by 385%. After the incubation of VSMC in 10-7 M PAMP for 24 h, ADM in the medium and ADM mRNA in the VSMC were decreased by 12.2 and 39.1%, respectively, and cAMP concentration in the VSMC was increased by 67%. The decreased ADM mRNA in VSMC induced by the ADM and PAMP treatment was completely reversed by the pre-treatment of the cells in 10(-7) M protein kinase inhibitor for 30 min. After the incubation of VSMC in 10(-7) M preproADM(153-185) (ADT) for 24 h, however, ADM in the medium and ADM mRNA in the VSMC were increased by 21 and 35.2%. The increased ADM mRNA in VSMC induced by the ADT treatment was partially blocked by the co-incubation in ADM and ADT, and was totally blocked by the co-incubation in PAMP + ADM and ADT, but was not blocked by the co-incubation in PAMP and ADT. Our results suggest that the four peptides derived from proadrenomedullin may have different effects, possibly through a cAMP-dependent pathway, on the expression of ADM gene. (C) 2002 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000177711200017
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58771
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1st, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Inst Cardiovasc Res, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Qi, YF,Bu, DF,Di Niu, D,et al. Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene[J]. PEPTIDES,2002,23(6):1141-1147.
APA Qi, YF.,Bu, DF.,Di Niu, D.,Shi, YR.,Wang, SH.,...&Du, JBA.(2002).Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene.PEPTIDES,23(6),1141-1147.
MLA Qi, YF,et al."Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene".PEPTIDES 23.6(2002):1141-1147.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qi, YF]的文章
[Bu, DF]的文章
[Di Niu, D]的文章
百度学术
百度学术中相似的文章
[Qi, YF]的文章
[Bu, DF]的文章
[Di Niu, D]的文章
必应学术
必应学术中相似的文章
[Qi, YF]的文章
[Bu, DF]的文章
[Di Niu, D]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。