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学科主题: 临床医学
题名:
Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene
作者: Qi, YF; Bu, DF; Di Niu, D; Shi, YR; Wang, SH; Pang, YZ; Tang, CS; Du, JBA
关键词: proadrenomedullin N-terminal 20 peptide (PAMP) ; adrenomedullin (ADM) ; adrenotensin (ADT) ; adrenomedullin gene
刊名: PEPTIDES
发表日期: 2002-06-01
卷: 23, 期:6, 页:1141-1147
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: VASCULAR SMOOTH-MUSCLE ; COMPETITIVE PCR ; CELLS ; 20-PEPTIDE ; PROTEIN
英文摘要:

Primary culture of vascular smooth muscle cells (VSMC) from rat aorta was used for the study of the effect of different peptides derived from proadrenomedullin on the expression of adrenomedullin (ADM) gene. ADM and preproADM(22-41) (PAMP) secreted by VSMC were measured by radioimmunoassay, and ADM mRNA in VSMC was determined by quantitative RT-PCR. After the incubation of VSMC in 10(-7) M ADM for 24 h, PAMP in the medium and ADM mRNA in the VSMC were decreased by 34 and 41.3%, respectively, and cAMP concentration in the VSMC was increased by 385%. After the incubation of VSMC in 10-7 M PAMP for 24 h, ADM in the medium and ADM mRNA in the VSMC were decreased by 12.2 and 39.1%, respectively, and cAMP concentration in the VSMC was increased by 67%. The decreased ADM mRNA in VSMC induced by the ADM and PAMP treatment was completely reversed by the pre-treatment of the cells in 10(-7) M protein kinase inhibitor for 30 min. After the incubation of VSMC in 10(-7) M preproADM(153-185) (ADT) for 24 h, however, ADM in the medium and ADM mRNA in the VSMC were increased by 21 and 35.2%. The increased ADM mRNA in VSMC induced by the ADT treatment was partially blocked by the co-incubation in ADM and ADT, and was totally blocked by the co-incubation in PAMP + ADM and ADT, but was not blocked by the co-incubation in PAMP and ADT. Our results suggest that the four peptides derived from proadrenomedullin may have different effects, possibly through a cAMP-dependent pathway, on the expression of ADM gene. (C) 2002 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000177711200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58771
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1st, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Inst Cardiovasc Res, Beijing 100083, Peoples R China

Recommended Citation:
Qi, YF,Bu, DF,Di Niu, D,et al. Effects of different peptide fragments derived from proadrenomedullin on gene expression of adrenomedullin gene[J]. PEPTIDES,2002,23(6):1141-1147.
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