|Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections|
|Zhang Yi-jie1; Chen Yu-shen3; Wang Zhan-wei2; Li Yu-qian1; Wang Da-xuan3; Shang Ying1; Fu Rong-rong1; Hu Ying-hui4; Geng Rong5; Wei Li-ping6; Yang Jing-ping7; Li Jia-shu8; Yu Qin9; Du Juan10; Gao Zhan-cheng1|
|关键词||serotype multilocus sequence typing antibiotic resistance Streptococcus pneumonia|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||PNEUMOCOCCAL CONJUGATE VACCINE ; SEROTYPE 19A ; ANTIMICROBIAL RESISTANCE ; MULTIPLEX PCR ; CEREBROSPINAL-FLUID ; GENETIC-STRUCTURE ; ASIAN COUNTRIES ; UNITED-STATES ; CHILDREN ; IDENTIFICATION|
Background Streptococcus pneumoniae (S. pneumoniae) is a major causative agent of severe infections, including sepsis, pneumonia, meningitis, and otitis media, and has become a major public health concern. We report the pneumococcal serotype and sequence type (ST) distribution, and antimicrobial resistance of 39 S. pneumoniae strains from seven hospitals in China.
Methods Blood/cerebrospinal fluid (CSF) and sputum isolates from patients were analyzed to determine S. pneumoniae serotypes by polynnerase chain reaction (PCR) and the Neufeld Quellung reaction, the multilocus sequence types (MLST) by PCR and sequencing, and susceptibility to antimicrobial agents by the VITEK Gram Positive Susceptibility Card.
Results A total of 39 isolates were collected including 21 blood/CSF and 18 sputum isolates. Conventional serotyping by the Quellung reaction required 749 reactions. In contrast, PCR based typing needed only 106 PCR reactions. The most frequent serotypes from the blood/CSF isolates were 14 (38.1%), 19A (14.3%), 23F (9.5%), and 18C (9.5%). In the sputum isolates the most frequent serotypes were 19F (33.3%), 23F (16.7%), 19A (11.1%), and 3 (11.1%). The incidence of penicillin resistance in the blood/CSF and sputum isolates was 66.7% and 55.6%, respectively. Statistical analysis showed that patients <= 5 years old had a higher resistance to penicillin when they compared with the patients years old (P=0.011). Serotypes 14, 19A and 19F were significantly associated with penicillin resistance (P <0.001). ST320, ST271, and ST876 isolates showed high resistant rates to several antibiotics including penicillin (P=0.006). All of the isolates of serotype 19A were resistant to both penicillin and erythromycin, and they were all multi-drug resistant (MDR) isolates.
Conclusions The specificity and sensitivity of multiplex-PCR are good, and this method represents a substantial savings of time and money, and can be widely used in the laboratory and clinical practice. Data from this research showed an extremely high prevalence of penicillin resistance and an increasing prevalence of multi-drug resistant (MDR) rate in S. pneumoniae. A distinctive emergence of serotype 19A was observed which was also associated with the increasing prevalence of antimicrobial resistance. Therefore, nationwide surveillance of pneumococcal resistance and serotypes is strongly warranted.
|项目编号||2006AA02Z4A9 ; 2012BA105B02 ; 2012ZX10004-206 ; 201202011|
|资助机构||Ministry of Science and Technology of China ; Ministry of Health of China (Special Fund for Healthscientific Research in the Public Interest Program)|
|作者单位||1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China|
2.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China
3.Fujian Med Univ, Fujian Prov Hosp, Dept Resp Med, Fuzhou 350001, Fujian, Peoples R China
4.Capital Med Univ, Beijing Childrens Hosp, Dept Resp Med, Beijing 100045, Peoples R China
5.Capital Med Univ, Beijing Childrens Hosp, Dept Emergency Med, Beijing 100045, Peoples R China
6.Guangzhou Med Univ, Dept Resp Med, Affiliated Hosp 3, Guangzhou 510150, Guangdong, Peoples R China
7.Inner Mongolia Med Coll, Affiliated Hosp 3, Dept Resp & Crit Care Med, Baotou 014030, Inner Mongolia, Peoples R China
8.Xuzhou Med Coll, Lianyungang Peoples Hosp 1, Dept Resp Med, Lianyungang 222002, Jiangsu, Peoples R China
9.Lan Zhou Univ, Hosp 1, Dept Resp Med, Lanzhou 730000, Gansu, Peoples R China
10.Guiyang Med Coll, Affiliated Hosp, Dept Resp Med, Guiyang 550004, Guizhou, Peoples R China
|Zhang Yi-jie,Chen Yu-shen,Wang Zhan-wei,et al. Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections[J]. CHINESE MEDICAL JOURNAL,2013,126(12):2296-2303.|
|APA||Zhang Yi-jie.,Chen Yu-shen.,Wang Zhan-wei.,Li Yu-qian.,Wang Da-xuan.,...&Gao Zhan-cheng.(2013).Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections.CHINESE MEDICAL JOURNAL,126(12),2296-2303.|
|MLA||Zhang Yi-jie,et al."Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections".CHINESE MEDICAL JOURNAL 126.12(2013):2296-2303.|