IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer
Shao, Duan-Fang1; Wang, Xiao-Hong2; Li, Zi-Yu1; Xing, Xiao-Fang3; Cheng, Xiao-Jing3; Guo, Ting3; Du, Hong3; Hu, Ying2; Dong, Bin4; Ding, Ning1; Li, Lin1; Li, Shen1; Li, Qing-Da1; Wen, Xian-Zi3; Zhang, Lian-Hai1; Ji, Jia-Fu1
关键词SAE2 gastric cancer prognosis FoxM1 c-MYC
刊名AMERICAN JOURNAL OF CANCER RESEARCH
2015
5期:2页:589-602
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CELL-CYCLE ; SUMOYLATION ; SUMO ; PROGRESSION ; METASTASIS ; TRANSCRIPTION ; EXPRESSION ; SURVIVAL ; INVASION ; PROTEIN
英文摘要

Background: The SUMO pathway has been shown to play an important role in tumorigenesis. This report analyzed the involvement of the sole SUMO-Activating Enzyme Subunit 2 (SAE2) in human gastric cancer (GC) progression and prognosis. Methods: Expression of SAE2 was examined by Quantigene Plex, western blotting and immunohistochemistry. The expression of SAE2 and c-MYC were detected in parallel in 276 cases. The molecular mechanisms of SAE2 expression and its effects on cell growth, colony formation, migration and invasion were also explored by CCK8 assay, colony formation experiment, transwell chamber assay with or without matrigel, immuno-precipitation and in vivo tumorigenesis and tumor metastasis. Results: SAE2 was markedly overexpressed in GC cell lines and primary tumor samples of GC, and significantly correlated with deeper tumor depth, distant metastasis, higher pathological stage and stratified survival in human GC. SAE2 positivity was independently associated with a worse outcome in multivariate analysis. Knockdown of SAE2 expression inhibited the proliferation, migration, and invasion of SAE2-overexpressing GC cells. Consistent with the in vitro results, down-regulation of SAE2 in human GC BGC823 cells significantly reduced the tumorigenic and metastatic potential of the cells in vivo. SAE2 protein was significantly associated with the higher expression of c-MYC in primary GC tissues. Moreover, FoxM1 was SUMOylated in GC and that inhibition of SAE2 resulted in a decrease in SUMO1-FoxM1 levels compared with those in the controls. Conclusions: These findings suggest that SAE2 has a pivotal role in the aggressiveness of GC, and highlight its usefulness as a prognostic factor in GC.

语种英语
WOS记录号WOS:000352204800009
项目编号81101879 ; 2012AA02A203-B01 ; 2012AA02A-504-B01 ; 2012AA020101 ; Z121100007512010 ; D131100005313010
资助机构National Na-tural Science Foundation of China ; National Key Technology RD Program ; Beijing Municipal Science &amp ; Technology Commission
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58796
专题北京大学临床肿瘤学院
北京大学第二临床医学院_国际合作办公室
北京大学临床肿瘤学院_胃肠肿瘤中心一病区
北京大学临床肿瘤学院_中心实验室
北京大学临床肿瘤学院_603课题组
北京大学临床肿瘤学院_标本库
作者单位1.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Gastrointestinal Su, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Tissue Bank, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Gastrointestinal Tr, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
4.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Pathol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shao, Duan-Fang,Wang, Xiao-Hong,Li, Zi-Yu,et al. High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer[J]. AMERICAN JOURNAL OF CANCER RESEARCH,2015,5(2):589-602.
APA Shao, Duan-Fang.,Wang, Xiao-Hong.,Li, Zi-Yu.,Xing, Xiao-Fang.,Cheng, Xiao-Jing.,...&Ji, Jia-Fu.(2015).High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer.AMERICAN JOURNAL OF CANCER RESEARCH,5(2),589-602.
MLA Shao, Duan-Fang,et al."High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer".AMERICAN JOURNAL OF CANCER RESEARCH 5.2(2015):589-602.
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