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学科主题: 公共卫生
题名:
Integrin alpha(v)beta(6)-Targeted SPECT Imaging for Pancreatic Cancer Detection
作者: Liu, Zhaofei1,2; Liu, Hao1,2; Ma, Teng1,2; Sun, Xianlei1,2; Shi, Jiyun1; Jia, Bing1,2; Sun, Yi3; Zhan, Jun4,5; Zhang, Hongquan4,5; Zhu, Zhaohui3; Wang, Fan1,2,6
关键词: pancreatic cancer ; molecular imaging ; integrin alpha(v)beta(6) ; phage display ; SPECT/CT
刊名: JOURNAL OF NUCLEAR MEDICINE
发表日期: 2014-06-01
DOI: 10.2967/jnumed.113.132969
卷: 55, 期:6, 页:989-994
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: CYSTINE KNOT PEPTIDES ; IN-VIVO ; ALPHA-V-BETA-6 INTEGRIN ; MONOCLONAL-ANTIBODY ; COLON-CANCER ; LUNG-CANCER ; INTEGRIN-ALPHA(V)BETA(6) ; CARCINOMA ; CELLS ; RADIOPHARMACEUTICALS
英文摘要:

lntegrin alpha(v)beta(6), a member of the integrin family, is specifically expressed in many malignancies but not in normal organs. Overexpression of integrin alpha(v)beta(6) is usually correlated with malignant potential and poor prognosis. In this study, we describe the synthesis and evaluation of a Tc-99m-labeled integrin alpha(v)beta(6)-targeting peptide as a SPECT radiotracer for the in vivo imaging of integrin alpha(v)beta(6) expression. Methods: An integrin alpha(v)beta(6)-targeting peptide (denoted as the HK peptide) was conjugated with 6-hydrazinonicotinyl (HYNIC) and radiolabeled with Tc-99m using tricine and TPPTS (trisodium triphenylphosphine-3,3′,3 ′′ trisulfonate) as coligands. The in vitro and in vivo characteristics of Tc-99m-HYNIC(tricine)(TPPTS)-HK (Tc-99m-HHK) were investigated in BxPC-3 (integrin alpha(v)beta(6)-positive) and HEK293 (integrin alpha(v)beta(6)-negative) models. The ability of Tc-99m-HHK to detect liver metastasis of pancreatic cancer was evaluated using small-animal SPEC/CT. Results: Tc-99m-HHK showed high integrin alpha(v)beta(6)-binding specificity both in vitro and in vivo. Tc-99m-HHK was cleared rapidly from the blood and normal organs except for the kidneys. The highest uptake (0.88 +/- 0.12 percentage injected dose per gram) of Tc-99m-HHK in BxPC-3 tumors was observed at 0.5 h after injection. High-contrast images of integrin alpha(v)beta(6)-positive tumors were obtained using Tc-99m-HHK. The minimum nonspecific activity accumulation in normal liver tissues rendered high-quality SPECT/CT images of metastatic lesions. Conclusion: Tc-99m-HHK is a promising SPECT radiotracer for the noninvasive imaging of integrin alpha(v)beta(6) expression in vivo. SPECT/ CT with Tc-99m-HHK could provide an effective approach for the noninvasive detection of primary and metastatic lesions of integrin alpha(v)beta(6)-positive tumors. a

语种: 英语
所属项目编号: 81222019 ; 81125011 ; 81000625 ; 81201127 ; 81321003 ; 81230051 ; 2013CB733802 ; 2011CB707705 ; 2011YQ030114 ; 2012ZX09102301-018 ; 2012BAK25B03-16 ; 31300 ; BMU20110263 ; 7132131 ; 7132123 ; Z121107002512010
项目资助者: National Natural Science Foundation of China (NSFC) ; "973" projects ; Ministry of Science and Technology of China ; Ministry of Education of China ; Beijing Natural Science Foundation ; Beijing Nova Program
WOS记录号: WOS:000336829900023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58799
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Radiat Med, Beijing 100191, Peoples R China
3.Beijing Union Med Coll Hosp, Dept Nucl Med, Beijing, Peoples R China
4.Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Anat Histol & Embryol, Lab Mol Cell Biol & Tumor Biol, Beijing 100191, Peoples R China
6.Chinese Acad Sci, Inst Biophys, Interdisciplinary Lab, Beijing 100080, Peoples R China

Recommended Citation:
Liu, Zhaofei,Liu, Hao,Ma, Teng,et al. Integrin alpha(v)beta(6)-Targeted SPECT Imaging for Pancreatic Cancer Detection[J]. JOURNAL OF NUCLEAR MEDICINE,2014,55(6):989-994.
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