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Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples
Sun, Jiufeng1; Li, Xiqing1; Zeng, Hanxiang1; Xie, Zhi1; Lu, Changming1; Xi, Liyan1; de Hoog, Gert S.2,3,4
关键词Penicillium marneffei penicilliosis rapid diagnosis LAMP
刊名FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
2010-04-01
DOI10.1111/j.1574-695X.2009.00647.x
58期:3页:381-388
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases ; Microbiology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]ACUTE RESPIRATORY SYNDROME ; MYCOBACTERIUM-TUBERCULOSIS ; IDENTIFICATION ; ASSAY ; PCR ; HYBRIDIZATION ; DNA ; INFECTION ; YEAST ; CORONAVIRUS
英文摘要

Penicillium marneffei is the etiologic agent of a severe systemic disease in immunocompromised hosts in Southeast Asia. In the present study, a novel method, known as loop-mediated isothermal amplification (LAMP), is described for the rapid and specific detection of the species, using a primer set derived from the internal transcribed spacer (ITS) region of the rRNA gene. Amplification products can be detected macroscopically by visual inspection in vials using SYBR Green I as well as by electrophoresis on agarose gel. The LAMP assay resulted in specific amplification of P. marneffei ITS using pure cultures after a 1-h reaction at 65 degrees C in a water bath; no cross-reactivity with other fungi including other biverticillate penicillia was observed. The detectable DNA limit was two copies. In addition, specific amplification was achieved using paraffin wax-embedded tissue samples from patients with penicilliosis marneffei and tissue samples from bamboo rats. The method provides a powerful tool for rapid diagnostics in the clinical lab, and has potential for use in ecological studies.

语种英语
所属项目编号30770121/2007
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000275446600011
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58800
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Sun Yat Sen Univ, Affiliated Hosp 2, Dept Dermatol, Guangzhou 510120, Peoples R China
2.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
3.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands
4.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
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Sun, Jiufeng,Li, Xiqing,Zeng, Hanxiang,et al. Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples[J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY,2010,58(3):381-388.
APA Sun, Jiufeng.,Li, Xiqing.,Zeng, Hanxiang.,Xie, Zhi.,Lu, Changming.,...&de Hoog, Gert S..(2010).Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples.FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY,58(3),381-388.
MLA Sun, Jiufeng,et al."Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples".FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 58.3(2010):381-388.
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