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Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1
Huo, Li-Rong2; Ju, Weina; Yan, Ming2; Zou, Jun-Hua2; Yan, Wu2; He, Bing2; Zhao, Xin-Liang2; Jenkins, Edmund C.; Brown, W. Ted; Zhong, Nanbert1,2
关键词PCBP1 RNA interference Transcriptional profile Translational profile Histone proteins
刊名BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
2010-10-01
DOI10.1016/j.bbapap.2010.07.002
1804期:10页:1954-1964
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
资助者Chinese Ministry of Education (MOE) ; MOE ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD) ; Chinese Ministry of Education (MOE) ; MOE ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD)
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]POLY-C BINDING-PROTEIN-1 ; MESSENGER-RNA ; 3&prime ; -UNTRANSLATED REGION ; SIGNAL-TRANSDUCTION ; MOLECULAR-BASIS ; PROTEINS ; BINDING ; SUPPRESSION ; CANCER ; CELLS
英文摘要

PCBP1 is a member of the hnRNP family and participates in the regulation of transcription and translation. Previously, we identified transcripts targeted by overexpression of exogenous PCBP1. To further determine if these altered transcripts may also be targeted by a lack of PCBP1, we depleted endogenous PCBP1 in human SH-SY5Y cells. We identified 941 transcripts with the Affymetrix and 1362 with the Agilent expression platforms. There were 375 transcripts identified by both platforms, including 328 down-regulated and 47 up-regulated. The identified transcripts could be grouped into neuronal, cell signaling, metabolic, developmental, and differentiation categories, with pathway involvement in Wnt signaling, TGF beta signaling, translation factors and nuclear receptors. A proteomic profiling study with a two-dimensional chromatographic platform showed global translational changes over a range of isoelectric points (pI) = 4.84-8.42. This study identifies the transcripts affected by knock-down of endogenous PCBP1 and compares them to the transcripts affected by overexpression of PCBP1. (C) 2010 Elsevier B.V. All rights reserved.

语种英语
所属项目编号985-2035-39
资助者Chinese Ministry of Education (MOE) ; MOE ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD) ; Chinese Ministry of Education (MOE) ; MOE ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD)
WOS记录号WOS:000281920300002
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58805
专题基础医学院_北京大学医学遗传中心
作者单位1.Peking Univ, Ctr Med Genet, Beijing 100083, Peoples R China
2.New York State Inst Basic Res Dev Disabil, Dept Human Genet, Staten Isl, NY 10314 USA
推荐引用方式
GB/T 7714
Huo, Li-Rong,Ju, Weina,Yan, Ming,et al. Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2010,1804(10):1954-1964.
APA Huo, Li-Rong.,Ju, Weina.,Yan, Ming.,Zou, Jun-Hua.,Yan, Wu.,...&Zhong, Nanbert.(2010).Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1804(10),1954-1964.
MLA Huo, Li-Rong,et al."Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1804.10(2010):1954-1964.
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