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学科主题: 药学
题名:
Determination of echinacoside in rat serum by reversed-phase high-performance liquid chromatography with ultraviolet detection and its application to pharmacokinetics and bioavailability
作者: Jia, Cunqin; Shi, Haiming; Wu, Xiangmei; Li, Yinzeng; Chen, Jingjing; Tu, Pengfei
关键词: echinacoside ; pharmacokinetics ; bioavailability ; HPLC ; phenylethanoid glycoside
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2006-12-05
DOI: 10.1016/j.jchromb.2006.07.040
卷: 844, 期:2, 页:308-313
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PHENYLPROPANOID GLYCOSIDES ; PHENYLETHANOID GLYCOSIDES ; ANTIOXIDANT ACTIVITY ; PHENOLIC-COMPOUNDS ; ALKYLAMIDES
英文摘要:

A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of echinacoside (ECH) in rat serum. After protein precipitation of serum sample with trichloroacetic acid, the supernatant was directly injected and analyzed on a C-18 CapcellACR analytical column (150 mm x 4.6 mm I.D. 5 mu m) with a mobile phase consisting of acetonitrile-0.5% acetic acid (15.5:84.5, v/v). The UV detector was set at 330 nm. The lower limit of detection and quantification were 9 and 29.2 ng/mL, respectively, and the calibration curves were linear over the concentration range of 29.2-18250 ng/mL. The assay method was successfully applied to the study of the pharmacokinetics and bioavailability of ECH in rat. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000242843900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58809
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Jia, Cunqin,Shi, Haiming,Wu, Xiangmei,et al. Determination of echinacoside in rat serum by reversed-phase high-performance liquid chromatography with ultraviolet detection and its application to pharmacokinetics and bioavailability[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,844(2):308-313.
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