|Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium|
|Du Chan; Yang De-mei; Zhang Pei-xun; Deng Lei; Jiang Bao-guo|
|关键词||PC12 cells sciatic nerve optic nerve|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||GROWTH-FACTOR ; SCHWANN-CELLS ; SPINAL-CORD ; PERIPHERAL-NERVE ; MESSENGER-RNAS ; EXPRESSION ; RATS ; BINDING ; AXONS ; NEUROTROPHIN-3|
Background Previous work has shown that optic nerve and sciatic nerve conditional medium had neurotrophic activity on neurons. In order to find if the optic nerve conditioned media (CM) had a similar activity to make PC12 cells differentiate as sciatic nerve CM did, we explored the neurotrophic activity in optic nerve CM in the same in vitro system and compared the neurotrophin expression levels in optic and sciatic nerves under both conditions.
Methods PC12 cells were used to examine the effects of neurotrophins secreted by the sciatic nerve and optic nerve. RT-PCR and real-time QPCR showed that the sciatic nerve and optic nerve produced a range of neurotrophins including nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3).
Results The effects of sciatic nerve and optic nerve CM on neurite outgrowth were tested against a range of neurotrophins, and they had different neuritogenic activities. Only NGF and sciatic nerve CM had obvious neuritogenic activities, although the concentration of NGF in the sciatic nerve CM was very low.
Conclusions Our experiment showed that sciatic nerve CM had a higher neurotrophic activity on PC12 cells than optic nerve CM. These results suggested that peripheral nervous system (PNS) and central nervous system (CNS) had different expression levels of neurotrophin, which may in part explain the lack of ability to regenerate the CNS. Chin Med J 2010;123(3):351-355
|项目编号||30625036 ; 2005CB522604 ; 30801169 ; A-2008-10 ; 20070001780|
|资助机构||Chinese National Natural Science Fund for Outstanding Youth ; Chinese 973 Project Planning ; Chinese National Natural Science Youth Fund ; Beijng City Science and Technology New Star Classificantion ; Chinese Ministry of Education for Doctor Position New Teacher|
|作者单位||Peking Univ, Peoples Hosp, Dept Orthoped & Traumatol, Beijing 100044, Peoples R China|
|Du Chan,Yang De-mei,Zhang Pei-xun,et al. Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium[J]. CHINESE MEDICAL JOURNAL,2010,123(3):351-355.|
|APA||Du Chan,Yang De-mei,Zhang Pei-xun,Deng Lei,&Jiang Bao-guo.(2010).Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium.CHINESE MEDICAL JOURNAL,123(3),351-355.|
|MLA||Du Chan,et al."Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium".CHINESE MEDICAL JOURNAL 123.3(2010):351-355.|