IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium
Du Chan; Yang De-mei; Zhang Pei-xun; Deng Lei; Jiang Bao-guo
关键词PC12 cells sciatic nerve optic nerve
刊名CHINESE MEDICAL JOURNAL
2010-02-05
DOI10.3760/cma.j.issn.0366-6999.2010.03.017
123期:3页:351-355
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]GROWTH-FACTOR ; SCHWANN-CELLS ; SPINAL-CORD ; PERIPHERAL-NERVE ; MESSENGER-RNAS ; EXPRESSION ; RATS ; BINDING ; AXONS ; NEUROTROPHIN-3
英文摘要

Background Previous work has shown that optic nerve and sciatic nerve conditional medium had neurotrophic activity on neurons. In order to find if the optic nerve conditioned media (CM) had a similar activity to make PC12 cells differentiate as sciatic nerve CM did, we explored the neurotrophic activity in optic nerve CM in the same in vitro system and compared the neurotrophin expression levels in optic and sciatic nerves under both conditions.

Methods PC12 cells were used to examine the effects of neurotrophins secreted by the sciatic nerve and optic nerve. RT-PCR and real-time QPCR showed that the sciatic nerve and optic nerve produced a range of neurotrophins including nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3).

Results The effects of sciatic nerve and optic nerve CM on neurite outgrowth were tested against a range of neurotrophins, and they had different neuritogenic activities. Only NGF and sciatic nerve CM had obvious neuritogenic activities, although the concentration of NGF in the sciatic nerve CM was very low.

Conclusions Our experiment showed that sciatic nerve CM had a higher neurotrophic activity on PC12 cells than optic nerve CM. These results suggested that peripheral nervous system (PNS) and central nervous system (CNS) had different expression levels of neurotrophin, which may in part explain the lack of ability to regenerate the CNS. Chin Med J 2010;123(3):351-355

语种英语
WOS记录号WOS:000274753400017
项目编号30625036 ; 2005CB522604 ; 30801169 ; A-2008-10 ; 20070001780
资助机构Chinese National Natural Science Fund for Outstanding Youth ; Chinese 973 Project Planning ; Chinese National Natural Science Youth Fund ; Beijng City Science and Technology New Star Classificantion ; Chinese Ministry of Education for Doctor Position New Teacher
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58835
专题北京大学第二临床医学院
北京大学第二临床医学院_创伤骨科
北京大学第二临床医学院_党委院长办公室
作者单位Peking Univ, Peoples Hosp, Dept Orthoped & Traumatol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Du Chan,Yang De-mei,Zhang Pei-xun,et al. Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium[J]. CHINESE MEDICAL JOURNAL,2010,123(3):351-355.
APA Du Chan,Yang De-mei,Zhang Pei-xun,Deng Lei,&Jiang Bao-guo.(2010).Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium.CHINESE MEDICAL JOURNAL,123(3),351-355.
MLA Du Chan,et al."Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium".CHINESE MEDICAL JOURNAL 123.3(2010):351-355.
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