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学科主题: 临床医学
题名:
Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium
作者: Du Chan; Yang De-mei; Zhang Pei-xun; Deng Lei; Jiang Bao-guo
关键词: PC12 cells ; sciatic nerve ; optic nerve
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-02-05
DOI: 10.3760/cma.j.issn.0366-6999.2010.03.017
卷: 123, 期:3, 页:351-355
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: GROWTH-FACTOR ; SCHWANN-CELLS ; SPINAL-CORD ; PERIPHERAL-NERVE ; MESSENGER-RNAS ; EXPRESSION ; RATS ; BINDING ; AXONS ; NEUROTROPHIN-3
英文摘要:

Background Previous work has shown that optic nerve and sciatic nerve conditional medium had neurotrophic activity on neurons. In order to find if the optic nerve conditioned media (CM) had a similar activity to make PC12 cells differentiate as sciatic nerve CM did, we explored the neurotrophic activity in optic nerve CM in the same in vitro system and compared the neurotrophin expression levels in optic and sciatic nerves under both conditions.

Methods PC12 cells were used to examine the effects of neurotrophins secreted by the sciatic nerve and optic nerve. RT-PCR and real-time QPCR showed that the sciatic nerve and optic nerve produced a range of neurotrophins including nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3).

Results The effects of sciatic nerve and optic nerve CM on neurite outgrowth were tested against a range of neurotrophins, and they had different neuritogenic activities. Only NGF and sciatic nerve CM had obvious neuritogenic activities, although the concentration of NGF in the sciatic nerve CM was very low.

Conclusions Our experiment showed that sciatic nerve CM had a higher neurotrophic activity on PC12 cells than optic nerve CM. These results suggested that peripheral nervous system (PNS) and central nervous system (CNS) had different expression levels of neurotrophin, which may in part explain the lack of ability to regenerate the CNS. Chin Med J 2010;123(3):351-355

语种: 英语
所属项目编号: 30625036 ; 2005CB522604 ; 30801169 ; A-2008-10 ; 20070001780
项目资助者: Chinese National Natural Science Fund for Outstanding Youth ; Chinese 973 Project Planning ; Chinese National Natural Science Youth Fund ; Beijng City Science and Technology New Star Classificantion ; Chinese Ministry of Education for Doctor Position New Teacher
WOS记录号: WOS:000274753400017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58835
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Orthoped & Traumatol, Beijing 100044, Peoples R China

Recommended Citation:
Du Chan,Yang De-mei,Zhang Pei-xun,et al. Neuronal differentiation of PC12 cells induced by sciatic nerve and optic nerve conditioned medium[J]. CHINESE MEDICAL JOURNAL,2010,123(3):351-355.
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