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Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells
Jiang, Ning1,2,3; Zhou, Li-Qun1,2,3; Zhang, Xiao-Yu1,2,3
关键词apoptosis NSBP1 proliferation prostate cancer RNA interference
刊名ASIAN JOURNAL OF ANDROLOGY
2010-09-01
DOI10.1038/aja.2010.39
12期:5页:709-717
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]CHROMOSOMAL-PROTEINS ; HIGH-MOBILITY ; HMGN PROTEINS ; APOPTOSIS ; CHROMATIN ; EXPRESSION ; DEATH ; CYCLE ; P53 ; RADIATION
英文摘要

This studay is to construct a lentiviral vector harbouring an RNA interference (RNAi) sequence that targets the gene encoding the human high-mobility group nucleosomal binding protein 1 (NSBP1); to study its role in inducing G(2)/M phase arrest and apoptosis in prostate cancer (PCa) DU145 cells; and to assess the effect of its knockdown on cell proliferation in vitro and in vivo. RNAi was applied to knock down NSBP1 expression in the PCa cell line DU145 by lentiviral plasmids producing an NSBP1 small hairpin RNA. After NSBP1 knockdown in DU145 cells, the growth rate of cells was analyzed by MTT, and G(2)/M cell cycle arrest and apoptosis were assessed using a FAC-Scalibur flow cytometer. Tumour growth was assessed in nude mice. The mRNA and protein expression levels of NSBP1, cyclin B1 and Bcl-2 were analysed in vitro and in vivo by reverse-transcriptase polymerase chain reaction and Western blotting. Knockdown of NSBP1 resulted in a 22.6% decrease in the growth rate of cells compared with the PscNC lentivirus control cells at 96 h, decreased tumour growth in nude mice, and the induction of G(2)/M cell cycle arrest (8.78%) and apoptosis (2.19-fold). Consistent with the cell cycle arrest and apoptosis, the mRNA and protein expression levels of cyclin B1 and Bcl-2 were decreased. In conclusion, knockdown of NSBP1 causes a statistically significant inhibition of the in vitro and in vivo growth of the PCa cell line DU145. Growth suppression is at least partially due to NSBP1 knockdown-induced G(2)/M cell cycle arrest and apoptosis. The present data provide the evidence that the NSBP1 knockdown-induced G(2)/M phase arrest and apoptosis may result from negative regulation of cyclin B1 and Bcl-2 by NSBP1, with the resulting reduced expression of these proteins.

语种英语
WOS记录号WOS:000281602200012
项目编号30271295 ; 30672099 ; 7092101
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation of China
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58841
专题北京大学第一临床医学院_泌尿外科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Ning,Zhou, Li-Qun,Zhang, Xiao-Yu. Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(5):709-717.
APA Jiang, Ning,Zhou, Li-Qun,&Zhang, Xiao-Yu.(2010).Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells.ASIAN JOURNAL OF ANDROLOGY,12(5),709-717.
MLA Jiang, Ning,et al."Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells".ASIAN JOURNAL OF ANDROLOGY 12.5(2010):709-717.
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