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学科主题: 临床医学
题名:
Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells
作者: Jiang, Ning1,2,3; Zhou, Li-Qun1,2,3; Zhang, Xiao-Yu1,2,3
关键词: apoptosis ; NSBP1 ; proliferation ; prostate cancer ; RNA interference
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2010-09-01
DOI: 10.1038/aja.2010.39
卷: 12, 期:5, 页:709-717
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: CHROMOSOMAL-PROTEINS ; HIGH-MOBILITY ; HMGN PROTEINS ; APOPTOSIS ; CHROMATIN ; EXPRESSION ; DEATH ; CYCLE ; P53 ; RADIATION
英文摘要:

This studay is to construct a lentiviral vector harbouring an RNA interference (RNAi) sequence that targets the gene encoding the human high-mobility group nucleosomal binding protein 1 (NSBP1); to study its role in inducing G(2)/M phase arrest and apoptosis in prostate cancer (PCa) DU145 cells; and to assess the effect of its knockdown on cell proliferation in vitro and in vivo. RNAi was applied to knock down NSBP1 expression in the PCa cell line DU145 by lentiviral plasmids producing an NSBP1 small hairpin RNA. After NSBP1 knockdown in DU145 cells, the growth rate of cells was analyzed by MTT, and G(2)/M cell cycle arrest and apoptosis were assessed using a FAC-Scalibur flow cytometer. Tumour growth was assessed in nude mice. The mRNA and protein expression levels of NSBP1, cyclin B1 and Bcl-2 were analysed in vitro and in vivo by reverse-transcriptase polymerase chain reaction and Western blotting. Knockdown of NSBP1 resulted in a 22.6% decrease in the growth rate of cells compared with the PscNC lentivirus control cells at 96 h, decreased tumour growth in nude mice, and the induction of G(2)/M cell cycle arrest (8.78%) and apoptosis (2.19-fold). Consistent with the cell cycle arrest and apoptosis, the mRNA and protein expression levels of cyclin B1 and Bcl-2 were decreased. In conclusion, knockdown of NSBP1 causes a statistically significant inhibition of the in vitro and in vivo growth of the PCa cell line DU145. Growth suppression is at least partially due to NSBP1 knockdown-induced G(2)/M cell cycle arrest and apoptosis. The present data provide the evidence that the NSBP1 knockdown-induced G(2)/M phase arrest and apoptosis may result from negative regulation of cyclin B1 and Bcl-2 by NSBP1, with the resulting reduced expression of these proteins.

语种: 英语
所属项目编号: 30271295 ; 30672099 ; 7092101
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation of China
WOS记录号: WOS:000281602200012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58841
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Natl Urol Canc Ctr, Beijing 100034, Peoples R China

Recommended Citation:
Jiang, Ning,Zhou, Li-Qun,Zhang, Xiao-Yu. Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(5):709-717.
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