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学科主题: 基础医学
题名:
In vivo tracking of implanted stem cells using radio-labeled transferrin scintigraphy
作者: Ding, W; Bai, J; Zhang, J; Chen, Y; Cao, L; He, Y; Shen, L; Wang, F; Tian, J
关键词: radionuclide imaging ; stein cells ; transplantation ; transferrin receptors ; spinal cord
刊名: NUCLEAR MEDICINE AND BIOLOGY
发表日期: 2004-08-01
DOI: 10.1016/j.nucmedbio.2004.04.001
卷: 31, 期:6, 页:719-725
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM ; SPINAL-CORD ; RAT ; RECEPTORS ; TRANSPLANTATION ; BLOOD ; BRAIN
英文摘要:

The possibility of monitoring stein cells in vivo with radionuclide imaging after transplantation was investigated. Based on the results of a radioligand receptors assay that human mesenchymal stein cells (hMSCs) express a high level of transferrin receptors, iodinated transferrin (I-131-Tf(Fe)(2)) was chosen as the radiotracer for imaging the cells implanted into the spinal cords of rabbits. Accumulation of radioactivity at the cell transplanted sites was assessed 16 and 24 hours post-intrathecal injection of I-131-Tf(Fe)(2). Transferrin receptors expression and Tf binding of the implanted cells were verified by immunofluorescence, and ex vivo phosphor imaging. The specificity of Tf uptake of hMSCs was proved through control experiments, i.e., replacing I-131-Tf(Fe), with I-131 labeled human serum albumin as the tracer or substituting hMSCs with phosphate buffered saline as the grafts. Despite some defects, such as the invasive administration of the tracer and the non-specificity of transferrin receptors as a marker of stem cells in this preliminary study, the technique of nuclear medicine imaging is considered to have great potential in tracking implanted cells in vivo. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000222882600005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58845
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Chinese Peoples Liberat Army Gen Hosp, Dept Nucl Med, Beijing, Peoples R China
2.Peking Univ, Dept Orthoped, Beijing, Peoples R China
3.Peking Univ, Stem Cell Res Ctr, Beijing, Peoples R China
4.Peking Univ, Med Isotopes Res Ctr, Beijing, Peoples R China

Recommended Citation:
Ding, W,Bai, J,Zhang, J,et al. In vivo tracking of implanted stem cells using radio-labeled transferrin scintigraphy[J]. NUCLEAR MEDICINE AND BIOLOGY,2004,31(6):719-725.
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