|Gene Decay in Shigella as an Incipient Stage of Host-Adaptation|
|Feng, Ye1; Chen, Zhe2; Liu, Shu-Lin1,3,4|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||ESCHERICHIA-COLI K-12 ; MOLECULAR EVOLUTIONARY HISTORY ; COMPLETE GENOME SEQUENCE ; MAXIMUM-LIKELIHOOD ; FLEXNERI 2A ; DYNAMICS ; PATHOGENICITY ; DIVERSITY ; VIRULENCE ; INSIGHTS|
Background: Many facultative bacterial pathogens have undergone extensive gene decay processes, possibly due to lack of selection pressure during evolutionary conversion from free-living to intracellular lifestyle. Shigella, the causative agents of human shigellosis, have arisen from different E. coli-like ancestors independently by convergent paths. As these bacteria all have lost large numbers of genes by mutation or deletion, they can be used as ideal models for systematically studying the process of gene function loss in different bacteria living under similar selection pressures.
Methodologies/Principal Findings: We compared the sequenced Shigella genomes and re-defined decayed genes (pseudogenes plus deleted genes) in these bacteria. Altogether, 85 genes are commonly decayed in the five analyzed Shigella strains and 1456 genes are decayed in at least one Shigella strain. Genes coding for carbon utilization, cell motility, transporter or membrane proteins are prone to be inactivated. Decayed genes tend to concentrate in certain operons rather than distribute averagely across the whole genome. Genes in the decayed operon accumulated more non-synonymous mutations than the rest genes and meanwhile have lower expression levels.
Conclusions: Different Shigella lineages underwent convergent gene decay processes, and inactivation of one gene would lead to a lesser selection pressure for the other genes in the same operon. The pool of superfluous genes for Shigella may contain at least two thousand genes and the gene decay processes may still continue in Shigella until a minimum genome harboring only essential genes is reached.
|项目编号||NSFC30870098 ; 30970119 ; 81030029 ; 20092307110001|
|资助机构||Harbin Medical University ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP)|
|作者单位||1.Harbin Med Coll, Genom Res Ctr, Harbin, Peoples R China|
2.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
3.Zhejiang Hosp Tradit Chinese Med, Natl Clin Res Base, Hangzhou, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
|Feng, Ye,Chen, Zhe,Liu, Shu-Lin. Gene Decay in Shigella as an Incipient Stage of Host-Adaptation[J]. PLOS ONE,2011,6(11).|
|APA||Feng, Ye,Chen, Zhe,&Liu, Shu-Lin.(2011).Gene Decay in Shigella as an Incipient Stage of Host-Adaptation.PLOS ONE,6(11).|
|MLA||Feng, Ye,et al."Gene Decay in Shigella as an Incipient Stage of Host-Adaptation".PLOS ONE 6.11(2011).|
|Gene Decay in Shigel（141KB）||期刊论文||出版稿||开放获取||CC BY-NC-SA||浏览 请求全文|