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学科主题: 临床医学
题名:
Enhanced EJ Cell Killing of I-125 Radiation by Combining with Cytosine Deaminase Gene Therapy Regulated by Synthetic Radio-Responsive Promoter
作者: Li, Ling1; Zhang, Chun-li1,2; Kang, Lei1; Wang, Rong-Fu1; Yan, Ping1; Zhao, Qian1; Yin, Lei1; Guo, Feng-qin1
关键词: 5-fluorocytosine ; I-125 ; cytosine deaminase ; radiation-sensitive promoter ; recombinant lentivirus
刊名: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
发表日期: 2015-10-01
DOI: 10.1089/cbr.2015.1862
卷: 30, 期:8, 页:342-348
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: RECEPTOR RADIONUCLIDE THERAPY ; IN-VITRO ; IONIZING-RADIATION ; TARGETED DIAGNOSIS ; EGR-1 PROMOTER ; EXPRESSION ; CANCER ; CARCINOMA ; TUMORS ; VIVO
英文摘要:

Aim: To investigate the enhancing effect of radionuclide therapy by the therapeutic gene placed under the control of radio-responsive promoter. Methods: The recombinant lentivirus E8-codA-GFP, including a synthetic radiation-sensitive promoter E8, cytosine deaminase (CD) gene, and green fluorescent protein gene, was constructed. The gene expression activated by I-125 radiation was assessed by observation of green fluorescence. The ability of converting 5-fluorocytosine (5-FC) to 5-fluorourial (5-FU) by CD enzyme was assessed by high-performance liquid chromatography. The viability of the infected cells exposed to I-125 in the presence of 5-FC was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the infected cells exposed to I-125 alone served as negative control and 5-FU as positive control. Results: The recombinant lentiviral vector was constructed successfully. On exposure of infected cells to I-125, green fluorescence can be observed and 5-FU can be detected. MTT assay showed that the survival rate for infected cells treated with I-125 was lower compared with the I-125 control group, but higher than the positive control group. Conclusion: The synthetic promoter E8 can induce the expression of downstream CD gene under I-125 radiation, and the tumor killing effect of I-125 can be enhanced by combining CD gene therapy with radiosensitive promoter.

语种: 英语
所属项目编号: 7083115 ; 7112129
项目资助者: Beijing Natural Science Foundation
WOS记录号: WOS:000362415200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58869
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

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作者单位: 1.Peking Univ, Dept Nucl Med, Hosp 1, Beijing 100034, Peoples R China
2.Jiangsu Inst Nucl Med, Key Lab Nucl Med, Jiangsu Key Lab Mol Nucl Med, Minist Hlth, Wuxi, Peoples R China

Recommended Citation:
Li, Ling,Zhang, Chun-li,Kang, Lei,et al. Enhanced EJ Cell Killing of I-125 Radiation by Combining with Cytosine Deaminase Gene Therapy Regulated by Synthetic Radio-Responsive Promoter[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2015,30(8):342-348.
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