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学科主题: 临床医学
题名:
The relationship among nephrin, podocin, CD2AP, and alpha-actinin might not be a true ′interaction′ in podocyte
作者: Fan, Q; Xing, Y; Ding, J; Guan, N; Zhang, J
关键词: RNAi ; mouse podocyte clone ; nephrin ; podocin ; CD2AP ; alpha-actinin-4
刊名: KIDNEY INTERNATIONAL
发表日期: 2006-04-01
DOI: 10.1038/sj.ki.5000245
卷: 69, 期:7, 页:1207-1215
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: PUROMYCIN AMINONUCLEOSIDE NEPHROSIS ; EXPERIMENTAL MEMBRANOUS NEPHROPATHY ; GLOMERULAR SLIT DIAPHRAGM ; RNA INTERFERENCE ; MAMMALIAN-CELLS ; SEGMENTAL GLOMERULOSCLEROSIS ; MOUSE PODOCYTE ; EXPRESSION ; MICE ; PROTEIN
英文摘要:

The abnormality of a single podocyte molecule, caused by a single gene mutation, such as NPHS1, NPHS2, CD2AP, and ACTN4, can lead to the hereditary/congenital nephrotic syndromes (NS). Further studies suggested that more than one podocyte molecule were together involved in acquired or experimental NS. However, we do not know much on the relationship among these podocyte molecules, and the molecular response induced by the change of each podocyte protein to the remaining ones. We respectively knockdown the nephrin, podocin, CD2AP, or alpha-actinin-4 mRNA by using reconstructed RNA interference vector-psiRNA-hH1GFPzeo in mouse podocyte clone. The molecular behavior or response was revealed by the quantitative expression both at mRNA and protein levels with RT-PCR and Western blot, and by the molecular distribution detected with confocal microscopy. With nephrin knockdown, only CD2AP increased, whereas podocin showed no change. Contrarily, with podocin or CD2AP knockdown, nephrin decreased, while CD2AP or podocin increased. Nephrin, podocin, or CD2AP knockdown did not change the expression of alpha-actinin-4, whereas alpha-actinin-4 knockdown begetted the reduction of nephrin, and the increment of podocin and CD2AP. The redistributions of nephrin, podocin, and CD2AP were revealed around a predominant nuclear staining compared with the membrane surface staining in the control podocytes. Our data imply that the response between the four podocyte molecules is very complicated and evidently different. There is not always an interaction between podocyte molecules. The normal localization of podocyte molecules would depend on their normal expression quantity and the molecular reactions between them.

语种: 英语
WOS记录号: WOS:000236724000021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58886
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Fan, Q,Xing, Y,Ding, J,et al. The relationship among nephrin, podocin, CD2AP, and alpha-actinin might not be a true ′interaction′ in podocyte[J]. KIDNEY INTERNATIONAL,2006,69(7):1207-1215.
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