IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
alpha(1D)-Adrenergic receptor insensitivity is associated with alterations in its expression and distribution in cultured vascular myocytes
Fan, Lin-lin1; Ren, Shuang1; Zhou, Hong3; Wang, Ying2; Xu, Ping-xiang1; He, Jun-qi2; Luo, Da-li1
关键词alpha(1D)-adrenergic receptor vascular smooth muscle cells receptor sensitivity receptor distribution Ca2+ signaling
刊名ACTA PHARMACOLOGICA SINICA
2009-12-01
DOI10.1038/aps.2009.160
30期:12页:1585-1593
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SMOOTH-MUSCLE-CELLS ; ALPHA(1)-ADRENERGIC RECEPTORS ; BLOOD-PRESSURE ; CELLULAR-LOCALIZATION ; SIGNAL-TRANSDUCTION ; CA2+ ENTRY ; SUBTYPES ; HYPERTROPHY ; AORTA ; VASOCONSTRICTION
英文摘要

Aim: It is unclear why alpha(1D)-adrenergic receptors (alpha(1D)-ARs) play a critical role in the mediation of peripheral vascular resistance and blood pressure in situ but function inefficiently when studied in vitro. The present study examined the causes for these inconsistencies in native alpha(1)-adrenergic functional performance between the vascular smooth muscle and myocytes.

Methods: The alpha(1)-adrenergic mediated contraction, Ca2+ signaling and the subcellular receptor distribution were evaluated using the Fluo-4, BODIPY-FL prazosin and subtype-specific antibodies.

Results: Rat aortic rings and freshly dissociated myocytes displayed contractile and increased intracellular Ca2+ responses to stimulation with phenylephrine (PE, 10 mu mol), respectively. However, the PE-induced responses disappeared completely in cultured aortic myocytes, whereas PE-enhanced Ca2+ transients were seen in cultured rat cardiac myocytes. Further studies indicated that alpha(1D)-ARs, the major receptor subtype responsible for the alpha(1)-adrenergic regulation of aortic contraction, were distributed both intracellularly and at the cell membrane in freshly dispersed aortic myocytes, similar to the alpha(1A)-AR subcellular localization in the cultured cardiomyocytes. In the cultured aortic myocytes, however, in addition to a marked decrease in their protein expression relative to the aorta, most labeling signals for alpha(1D)-ARs were found in the cytoplasm. Importantly, treating the culture medium with charcoal/dextran caused the reappearance of alpha(1D)-ARs at the cell surface and a partial restoration of the Ca2+ signal response to PE in approximately 30% of the cultured cells.

Conclusion: Reduction in alpha(1D)-AR total protein expression and disappearance from the cell surface contribute to the insensitivity of cultured vascular smooth muscle cells to alpha(1)-adrenergic receptor activation.

语种英语
WOS记录号WOS:000273048500001
项目编号30772574 ; 7082018
资助机构National Natural Science Foundation ; Beijing Municipal ; Scientific Research Common Program of the Beijing Municipal Commission of Education ; Beijing Natural Science Foundation
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58889
专题北京大学基础医学院_药理学系
作者单位1.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
2.Capital Med Univ, Dept Biochem, Beijing 100069, Peoples R China
3.Peking Univ, Med Sci Ctr, Dept Pharmacol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Fan, Lin-lin,Ren, Shuang,Zhou, Hong,et al. alpha(1D)-Adrenergic receptor insensitivity is associated with alterations in its expression and distribution in cultured vascular myocytes[J]. ACTA PHARMACOLOGICA SINICA,2009,30(12):1585-1593.
APA Fan, Lin-lin.,Ren, Shuang.,Zhou, Hong.,Wang, Ying.,Xu, Ping-xiang.,...&Luo, Da-li.(2009).alpha(1D)-Adrenergic receptor insensitivity is associated with alterations in its expression and distribution in cultured vascular myocytes.ACTA PHARMACOLOGICA SINICA,30(12),1585-1593.
MLA Fan, Lin-lin,et al."alpha(1D)-Adrenergic receptor insensitivity is associated with alterations in its expression and distribution in cultured vascular myocytes".ACTA PHARMACOLOGICA SINICA 30.12(2009):1585-1593.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
alpha(1D)-Adrenergic(4587KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fan, Lin-lin]的文章
[Ren, Shuang]的文章
[Zhou, Hong]的文章
百度学术
百度学术中相似的文章
[Fan, Lin-lin]的文章
[Ren, Shuang]的文章
[Zhou, Hong]的文章
必应学术
必应学术中相似的文章
[Fan, Lin-lin]的文章
[Ren, Shuang]的文章
[Zhou, Hong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: alpha(1D)-Adrenergic receptor insensitivity is associated with alterations in its expression and distribution in cultured vascular myocytes.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。