北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 细胞生物学研究室  > 期刊论文
学科主题: 临床医学
题名:
Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin
作者: Ojima, K; Lin, ZX; Bang, ML; Holtzer, S; Matsuda, R; Labeit, S; Sweeney, HL; Holtzer, H
关键词: Z-bands ; myogenesis ; I-Z-I bands ; alpha-actin ; phalloidin staining
刊名: JOURNAL OF CELL BIOLOGY
发表日期: 2000-08-07
卷: 150, 期:3, 页:553-566
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: SARCOMERIC-ALPHA-ACTININ ; CULTURED CARDIAC MYOCYTES ; FIBER-LIKE STRUCTURES ; Z-I-BODIES ; SKELETAL-MUSCLE ; THIN-FILAMENTS ; Z-BAND ; 3-DIMENSIONAL STRUCTURE ; NASCENT MYOFIBRILS ; NONMUSCLE ISOFORMS
英文摘要:

To learn how nebulin functions in the assembly and maintenance of I-Z-I bands, MYC- and GFP-tagged nebulin fragments were expressed in primary cultured skeletal myotubes. Their sites of incorporation were visualized by double staining with anti-MYC, antibodies to myofibrillar proteins, and FITC- or rhodamine phalloidin. Contrary to expectations based on in vitro binding studies, none of the nebulin fragments expressed in maturing myotubes were incorporated selectively into I-band similar to 1.0-mu m F-alpha-actin-containing thin filaments. Four of the MYC/COOH-terminal nebulin fragments were incorporated exclusively into periodic similar to 0.1-mu m Z-bands. Whereas both anti-MYC and Rho-phalloidin stained intra-Z-band F-alpha-actin oligomers, only the latter stained the pointed ends of the polarized similar to 1.0-mu m thin filaments. Z-band incorporation was independent of the nebulin COOH-terminal Ser or SH3 domains. In vitro cosedimentation studies also demonstrated that nebulin SH3 fragments did not bind to F-alpha-actin or alpha-actinin. The remaining six fragments were not incorporated into Z-bands, but were incorporated (a) diffusely throughout the sarcoplasm and into (b) fibrils/patches of varying lengths and widths nested among normal striated myofibrils. Over time, presumably in response to the mediation of muscle-specific homeostatic controls, many of the ectopic MYC-positive structures were resorbed. None of the tagged nebulin fragments behaved as dominant negatives; they neither blocked the assembly nor induced the disassembly of mature striated myofibrils. Moreover, they were not cytotoxic in myotubes, as they were in the fibroblasts and presumptive myoblasts in the same cultures.

语种: 英语
WOS记录号: WOS:000088727800014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58891
Appears in Collections:北京大学临床肿瘤学院_细胞生物学研究室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Tokyo, Dept Life Sci, Tokyo 1538092, Japan
2.Univ Penn, Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
3.Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA
4.Beijing Med Univ, Dept Cell Biol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
5.European Mol Biol Lab, D-69012 Heidelberg, Germany
6.Univ Heidelberg, Klinikum Mannheim, Dept Anesthesiol & Intens Operat Care, Mannheim, Germany

Recommended Citation:
Ojima, K,Lin, ZX,Bang, ML,et al. Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin[J]. JOURNAL OF CELL BIOLOGY,2000,150(3):553-566.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ojima, K]'s Articles
[Lin, ZX]'s Articles
[Bang, ML]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ojima, K]‘s Articles
[Lin, ZX]‘s Articles
[Bang, ML]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace