IR@PKUHSC  > 北京大学临床肿瘤学院  > 细胞生物学研究室
学科主题临床医学
Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin
Ojima, K; Lin, ZX; Bang, ML; Holtzer, S; Matsuda, R; Labeit, S; Sweeney, HL; Holtzer, H
关键词Z-bands myogenesis I-Z-I bands alpha-actin phalloidin staining
刊名JOURNAL OF CELL BIOLOGY
2000-08-07
150期:3页:553-566
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]SARCOMERIC-ALPHA-ACTININ ; CULTURED CARDIAC MYOCYTES ; FIBER-LIKE STRUCTURES ; Z-I-BODIES ; SKELETAL-MUSCLE ; THIN-FILAMENTS ; Z-BAND ; 3-DIMENSIONAL STRUCTURE ; NASCENT MYOFIBRILS ; NONMUSCLE ISOFORMS
英文摘要

To learn how nebulin functions in the assembly and maintenance of I-Z-I bands, MYC- and GFP-tagged nebulin fragments were expressed in primary cultured skeletal myotubes. Their sites of incorporation were visualized by double staining with anti-MYC, antibodies to myofibrillar proteins, and FITC- or rhodamine phalloidin. Contrary to expectations based on in vitro binding studies, none of the nebulin fragments expressed in maturing myotubes were incorporated selectively into I-band similar to 1.0-mu m F-alpha-actin-containing thin filaments. Four of the MYC/COOH-terminal nebulin fragments were incorporated exclusively into periodic similar to 0.1-mu m Z-bands. Whereas both anti-MYC and Rho-phalloidin stained intra-Z-band F-alpha-actin oligomers, only the latter stained the pointed ends of the polarized similar to 1.0-mu m thin filaments. Z-band incorporation was independent of the nebulin COOH-terminal Ser or SH3 domains. In vitro cosedimentation studies also demonstrated that nebulin SH3 fragments did not bind to F-alpha-actin or alpha-actinin. The remaining six fragments were not incorporated into Z-bands, but were incorporated (a) diffusely throughout the sarcoplasm and into (b) fibrils/patches of varying lengths and widths nested among normal striated myofibrils. Over time, presumably in response to the mediation of muscle-specific homeostatic controls, many of the ectopic MYC-positive structures were resorbed. None of the tagged nebulin fragments behaved as dominant negatives; they neither blocked the assembly nor induced the disassembly of mature striated myofibrils. Moreover, they were not cytotoxic in myotubes, as they were in the fibroblasts and presumptive myoblasts in the same cultures.

语种英语
WOS记录号WOS:000088727800014
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58891
专题北京大学临床肿瘤学院_细胞生物学研究室
作者单位1.Univ Tokyo, Dept Life Sci, Tokyo 1538092, Japan
2.Univ Penn, Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
3.Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA
4.Beijing Med Univ, Dept Cell Biol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
5.European Mol Biol Lab, D-69012 Heidelberg, Germany
6.Univ Heidelberg, Klinikum Mannheim, Dept Anesthesiol & Intens Operat Care, Mannheim, Germany
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GB/T 7714
Ojima, K,Lin, ZX,Bang, ML,et al. Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin[J]. JOURNAL OF CELL BIOLOGY,2000,150(3):553-566.
APA Ojima, K.,Lin, ZX.,Bang, ML.,Holtzer, S.,Matsuda, R.,...&Holtzer, H.(2000).Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin.JOURNAL OF CELL BIOLOGY,150(3),553-566.
MLA Ojima, K,et al."Distinct families of Z-line targeting modules in the COOH-terminal region of nebulin".JOURNAL OF CELL BIOLOGY 150.3(2000):553-566.
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