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学科主题临床医学
MiR-23a in Amplified 19p13.13 Loci Targets Metallothionein 2A and Promotes Growth in Gastric Cancer Cells
An, Juan1,2; Pan, Yuanming1; Yan, Zhi1; Li, Wenmei1; Cui, Jiantao1; Yuan, Jiao3; Tian, Liqing3; Xing, Rui1; Lu, Youyong1
关键词miR-23a GASTRIC CANCER COPY NUMBER VARIATION MT2A
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2013-09-01
DOI10.1002/jcb.24565
114期:9页:2160-2169
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]COPY NUMBER VARIATION ; COMPARATIVE GENOMIC HYBRIDIZATION ; MICRORNA GENES ; IDENTIFICATION ; EXPRESSION ; CARCINOMA ; CANDIDATE ; PATTERNS ; MARKERS
英文摘要

Copy number variation (CNV) and abnormal expression of microRNAs (miRNAs) always lead to deregulation of genes in cancer, including gastric cancer (GC). However, little is known about how CNVs affect the expression of miRNAs. By integrating CNV and miRNA profiles in the same samples, we identified eight miRNAs (miR-1274a, miR-196b, miR-4298, miR-181c, miR-181d, miR-23a, miR-27a and miR-24-2) that were located in the amplified regions and were upregulated in GC. In particular, amplification of miR-23a-27a-24-2 cluster and miR-181c-181d cluster frequently occurred at 19p13.13 and were confirmed by genomic real-time PCR in another 25 paired GC samples. Moreover, in situ hybridization (ISH) experiments represented that mature miR-23a was increased in GCs (75.5%, 40/53) compared with matched normal tissues (28.6%, 14/49, P=0.001). Knocking down of miR-23a expression inhibited BGC823 cell growth in vitro and in vivo. In addition, the potential target genes of miR-23a were investigated by integration of mRNA profile and miRNA TargetScan predictions, we found that upregulation of miR-23a and downregulation of metallothionein 2A (MT2A) were detected simultaneously in 70% (7/10) of the miRNA and mRNA profiles. Furthermore, an inverse correlation between miR-23a and MT2A expression was detected in GCs and normal tissues. Through combining luciferase assay, we confirmed that MT2A is a potential target of miR-23a. In conclusion, these results suggest that integration of CNV-miRNA-mRNA profiling is a powerful tool for identifying molecular signatures, and that miR-23a might play a role in regulating MT2A expression in GC. J. Cell. Biochem. 114: 2160-2169, 2013. (c) 2013 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000327699500023
项目编号2006AA02A402 ; 2012AA02A504 ; 2004CB518708
资助机构National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program)
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58893
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_高技术实验室
作者单位1.Peking Univ, Canc Hosp Inst, Minist Educ, Lab Mol Oncol,Key Lab Carcinogenesis & Translat R, Beijing 100142, Peoples R China
2.Qinghai Univ, Dept Basic Med Sci, High Altitude Med Res Ctr, Xining 810001, Qinghai Provinc, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Lab Bioinformat & Noncoding RNA, Beijing 100101, Peoples R China
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GB/T 7714
An, Juan,Pan, Yuanming,Yan, Zhi,et al. MiR-23a in Amplified 19p13.13 Loci Targets Metallothionein 2A and Promotes Growth in Gastric Cancer Cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2013,114(9):2160-2169.
APA An, Juan.,Pan, Yuanming.,Yan, Zhi.,Li, Wenmei.,Cui, Jiantao.,...&Lu, Youyong.(2013).MiR-23a in Amplified 19p13.13 Loci Targets Metallothionein 2A and Promotes Growth in Gastric Cancer Cells.JOURNAL OF CELLULAR BIOCHEMISTRY,114(9),2160-2169.
MLA An, Juan,et al."MiR-23a in Amplified 19p13.13 Loci Targets Metallothionein 2A and Promotes Growth in Gastric Cancer Cells".JOURNAL OF CELLULAR BIOCHEMISTRY 114.9(2013):2160-2169.
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