|Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer|
|Li, Yilin1; Zhang, Xiaotian1; Ge, Sai1; Gao, Jing1; Gong, Jifang1; Lu, Ming1; Zhang, Qiyue1; Cao, Yanshuo1; Wang, Daisy Dandan2; Lin, Peter Ping2; Shen, Lin1|
|关键词||gastric cancer circulating tumor cells EpCAM-independent enrichment HER2 aneuploidy|
|WOS标题词||Science & Technology|
|类目[WOS]||Oncology ; Cell Biology|
|研究领域[WOS]||Oncology ; Cell Biology|
|关键词[WOS]||BREAST-CANCER ; EPITHELIAL-CELLS ; EXPRESSION ; RESISTANCE ; HER2 ; CARCINOMA|
BACKGROUND: Karyotyping and phenotyping of circulating tumor cells (CTCs) in therapeutic cancer patients is of particular clinical significance in terms of both identifying chemo-resistant CTC subtypes and understanding CTC evolution.
METHODS: The integrated subtraction enrichment (SET) and immunostaining-fluorescence in situ hybridization (iFISH) platform was applied to detect and characterize CTCs in patients with advanced gastric cancer (AGC). Status of human epidermal growth factor receptor 2 (HER2) expressing and aneuploidy of chromosome 8 in CTCs enriched from the patients was examined by SET-iFISH following clinical chemotherapy or HER2-targeted therapy. CellSearch system was applied as a reference control.
RESULTS: Phenotyping of CTCs in HER2 positive AGC patients demonstrated that HER2(+) CTCs could be effectively eliminated in response to HER2-targeted therapy. Karyotyping of CTCs indicated that distinct CTCs with different ploidies of chromosomes 8 in AGC patients correlated to either sensitivity or resistance of paclitaxel or cisplatin-based chemotherapy. Examination of the copy number of chromosome 8 in CTCs provides a potential approach for predicting chemotherapeutic efficacy and monitoring chemo-resistance.
CONCLUSIONS: Phenotyping and karyotyping of the enriched CTCs upon ploidy of chromosome 8 or HER2 expression is of clinical potential for monitoring chemoresistance and evaluating therapeutic efficacy for AGC patients.
|作者单位||1.Cytelligen, San Diego, CA USA|
2.Peking Univ, Canc Hosp & Inst, Dept GI Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
|Li, Yilin,Zhang, Xiaotian,Ge, Sai,et al. Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer[J]. ONCOTARGET,2014,5(16):6594-6602.|
|APA||Li, Yilin.,Zhang, Xiaotian.,Ge, Sai.,Gao, Jing.,Gong, Jifang.,...&Shen, Lin.(2014).Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer.ONCOTARGET,5(16),6594-6602.|
|MLA||Li, Yilin,et al."Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer".ONCOTARGET 5.16(2014):6594-6602.|