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IR@PKUHSC  > 北京大学临床肿瘤学院  > 胃肠肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer
作者: Li, Yilin1; Zhang, Xiaotian1; Ge, Sai1; Gao, Jing1; Gong, Jifang1; Lu, Ming1; Zhang, Qiyue1; Cao, Yanshuo1; Wang, Daisy Dandan2; Lin, Peter Ping2; Shen, Lin1
关键词: gastric cancer ; circulating tumor cells ; EpCAM-independent enrichment ; HER2 ; aneuploidy
刊名: ONCOTARGET
发表日期: 2014-08-30
卷: 5, 期:16, 页:6594-6602
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: BREAST-CANCER ; EPITHELIAL-CELLS ; EXPRESSION ; RESISTANCE ; HER2 ; CARCINOMA
英文摘要:

BACKGROUND: Karyotyping and phenotyping of circulating tumor cells (CTCs) in therapeutic cancer patients is of particular clinical significance in terms of both identifying chemo-resistant CTC subtypes and understanding CTC evolution.

METHODS: The integrated subtraction enrichment (SET) and immunostaining-fluorescence in situ hybridization (iFISH) platform was applied to detect and characterize CTCs in patients with advanced gastric cancer (AGC). Status of human epidermal growth factor receptor 2 (HER2) expressing and aneuploidy of chromosome 8 in CTCs enriched from the patients was examined by SET-iFISH following clinical chemotherapy or HER2-targeted therapy. CellSearch system was applied as a reference control.

RESULTS: Phenotyping of CTCs in HER2 positive AGC patients demonstrated that HER2(+) CTCs could be effectively eliminated in response to HER2-targeted therapy. Karyotyping of CTCs indicated that distinct CTCs with different ploidies of chromosomes 8 in AGC patients correlated to either sensitivity or resistance of paclitaxel or cisplatin-based chemotherapy. Examination of the copy number of chromosome 8 in CTCs provides a potential approach for predicting chemotherapeutic efficacy and monitoring chemo-resistance.

CONCLUSIONS: Phenotyping and karyotyping of the enriched CTCs upon ploidy of chromosome 8 or HER2 expression is of clinical potential for monitoring chemoresistance and evaluating therapeutic efficacy for AGC patients.

语种: 英语
所属项目编号: 81301323 ; 81172110 ; 2012AA 02A 504 ; Z11110706730000
项目资助者: NSFC ; National High Technology Research and Development Program of China ; Beijing Municipal Science &amp ; Technology Commission Program
WOS记录号: WOS:000347920100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58914
Appears in Collections:北京大学临床肿瘤学院_胃肠肿瘤中心_期刊论文

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作者单位: 1.Cytelligen, San Diego, CA USA
2.Peking Univ, Canc Hosp & Inst, Dept GI Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Li, Yilin,Zhang, Xiaotian,Ge, Sai,et al. Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer[J]. ONCOTARGET,2014,5(16):6594-6602.
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