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学科主题: 临床医学
题名:
Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro
作者: Zhou, Hao1; Li, Dandan1; Shi, Chen2; Xin, Ting3; Yang, Junjie1; Zhou, Ying1; Hu, Shunyin1; Tian, Feng1; Wang, Jing1; Chen, Yundai1
刊名: SCIENTIFIC REPORTS
发表日期: 2015-08-07
DOI: 10.1038/srep12898
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ELEVATION MYOCARDIAL-INFARCTION ; GLUCAGON-LIKE PEPTIDE-1 ; STRESS-INDUCED-APOPTOSIS ; CHEMOKINE RECEPTORS ; FUTURE-DEVELOPMENTS ; SIGNALING PATHWAY ; HYDROGEN-PEROXIDE ; SERUM DEPRIVATION ; PROGENITOR CELLS ; PROTEIN-KINASE
英文摘要:

Mesenchymal stem cells (MSC) are regarded as an attractive source of therapeutic stem cells for myocardial infarction. However, their limited self-renewal capacity, low migration capacity and poor viability after transplantation hamper the clinical use of MSC; thus, a strategy to enhance the biological functions of MSC is required. Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor agonist, exerts cell-protective effects on many types of cells. However, little information is available regarding the influence of Ex-4 on MSC. In our study, MSC were isolated from bone marrow and cultured in vitro. After treatment with Ex-4, MSC displayed a higher proliferative capacity, increased C-X-C motif receptor 4 (CXCR4) expression and an enhanced migration response. Moreover, in H2O2induced apoptosis, Ex-4 preserved mitochondrial function through scavenging ROS and balancing the expression of anti-and pro-apoptotic proteins, leading to the inhibition of the mitochondriadependent cell death pathways and increased cell survival. Moreover, higher phospho-Akt (p-Akt) expression was observed after Ex-4 intervention. However, blockade of the PI3K/Akt pathway with inhibitors suppressed the above cytoprotective effects of Ex-4, suggesting that the PI3K/Akt pathway is partly responsible for Ex-4-mediated MSC growth, mobilization and survival. These findings provide an attractive method of maximizing the effectiveness of MSC-based therapies in clinical applications.

语种: 英语
所属项目编号: 2011AA020101 ; 81270186 ; 81400229
项目资助者: National 863 High Technology RD Program ; National Natural Science Foundation of China
WOS记录号: WOS:000359117200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58943
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Tianjin First Cent Hosp, Dept Cardiol, Tianjin, Peoples R China
2.Beijing Canc Hosp, Dept Radiotherapy, Beijing, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Dept Cardiol, Beijing, Peoples R China

Recommended Citation:
Zhou, Hao,Li, Dandan,Shi, Chen,et al. Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro[J]. SCIENTIFIC REPORTS,2015,5.
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