IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro
Zhou, Hao1; Li, Dandan1; Shi, Chen2; Xin, Ting3; Yang, Junjie1; Zhou, Ying1; Hu, Shunyin1; Tian, Feng1; Wang, Jing1; Chen, Yundai1
刊名SCIENTIFIC REPORTS
2015-08-07
DOI10.1038/srep12898
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ELEVATION MYOCARDIAL-INFARCTION ; GLUCAGON-LIKE PEPTIDE-1 ; STRESS-INDUCED-APOPTOSIS ; CHEMOKINE RECEPTORS ; FUTURE-DEVELOPMENTS ; SIGNALING PATHWAY ; HYDROGEN-PEROXIDE ; SERUM DEPRIVATION ; PROGENITOR CELLS ; PROTEIN-KINASE
英文摘要

Mesenchymal stem cells (MSC) are regarded as an attractive source of therapeutic stem cells for myocardial infarction. However, their limited self-renewal capacity, low migration capacity and poor viability after transplantation hamper the clinical use of MSC; thus, a strategy to enhance the biological functions of MSC is required. Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor agonist, exerts cell-protective effects on many types of cells. However, little information is available regarding the influence of Ex-4 on MSC. In our study, MSC were isolated from bone marrow and cultured in vitro. After treatment with Ex-4, MSC displayed a higher proliferative capacity, increased C-X-C motif receptor 4 (CXCR4) expression and an enhanced migration response. Moreover, in H2O2induced apoptosis, Ex-4 preserved mitochondrial function through scavenging ROS and balancing the expression of anti-and pro-apoptotic proteins, leading to the inhibition of the mitochondriadependent cell death pathways and increased cell survival. Moreover, higher phospho-Akt (p-Akt) expression was observed after Ex-4 intervention. However, blockade of the PI3K/Akt pathway with inhibitors suppressed the above cytoprotective effects of Ex-4, suggesting that the PI3K/Akt pathway is partly responsible for Ex-4-mediated MSC growth, mobilization and survival. These findings provide an attractive method of maximizing the effectiveness of MSC-based therapies in clinical applications.

语种英语
WOS记录号WOS:000359117200001
项目编号2011AA020101 ; 81270186 ; 81400229
资助机构National 863 High Technology RD Program ; National Natural Science Foundation of China
引用统计
被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58943
专题北京大学临床肿瘤学院
作者单位1.Tianjin First Cent Hosp, Dept Cardiol, Tianjin, Peoples R China
2.Beijing Canc Hosp, Dept Radiotherapy, Beijing, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Dept Cardiol, Beijing, Peoples R China
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GB/T 7714
Zhou, Hao,Li, Dandan,Shi, Chen,et al. Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro[J]. SCIENTIFIC REPORTS,2015,5.
APA Zhou, Hao.,Li, Dandan.,Shi, Chen.,Xin, Ting.,Yang, Junjie.,...&Chen, Yundai.(2015).Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro.SCIENTIFIC REPORTS,5.
MLA Zhou, Hao,et al."Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro".SCIENTIFIC REPORTS 5(2015).
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