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学科主题基础医学
Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides
Huang, Yanyan1; Zhao, Rui1; Fu, Yabin2; Zhang, Qundan1; Xiong, Shaoxiang1; Li, Li2; Zhou, Rouli2; Liu, Guoquan1; Chen, Yi1
关键词affinity chromatography cancer cell imaging molecular recognition peptides
刊名CHEMBIOCHEM
2011-05-16
DOI10.1002/cbic.201100031
12期:8页:1209-1215
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]PERFORMANCE AFFINITY-CHROMATOGRAPHY ; ANTISENSE PEPTIDE ; SMALL MOLECULES ; HEPATOCELLULAR-CARCINOMA ; RECOGNITION ; TUMORS ; INHIBITORS ; LIBRARIES ; PROTEINS ; DELIVERY
英文摘要

Specific detection and in vivo tracing of cancer biomarkers are important for cancer analysis. In this work, a simple and effective strategy for developing peptide probes was established. Peptides were rationally designed by using an antisense peptide approach directed towards an extracellular fragment (EL2) of a novel tumor-related protein LAPTM4B. Positional-scanning and stepwise affinity screening was employed to obtain an optimal peptide AP2H (IHGHHIISVG). The dissociation constant between the two small peptides, AP2H and the target EL2, was 5.51 mu m under physiological conditions. Fluorescence imaging assays indicated that AP2H can recognize live hepatoma cells by targeting the LAPTM4B protein on the cell surface with high specificity, low cytotoxicity and desirable cell penetrability. Compared to negative control cells, AP2H could differentiate cells with different expression levels of LAPTM4B. The screened peptide probe for molecular signatures of cancer cells, based on targeting the LAPTM4B protein, has potential applications in cancer diagnosis and targetable drug delivery.

语种英语
WOS记录号WOS:000290862400011
项目编号20975102 ; 21075125 ; 2007CB714504 ; CMS-CX200912
资助机构National Natural Science Foundation of China ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Institute of Chemistry, CAS
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58952
专题北京大学基础医学院_细胞生物学系
作者单位1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Peking Univ, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Huang, Yanyan,Zhao, Rui,Fu, Yabin,et al. Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides[J]. CHEMBIOCHEM,2011,12(8):1209-1215.
APA Huang, Yanyan.,Zhao, Rui.,Fu, Yabin.,Zhang, Qundan.,Xiong, Shaoxiang.,...&Chen, Yi.(2011).Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides.CHEMBIOCHEM,12(8),1209-1215.
MLA Huang, Yanyan,et al."Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides".CHEMBIOCHEM 12.8(2011):1209-1215.
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