北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 细胞生物学系  > 期刊论文
学科主题: 基础医学
题名:
Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides
作者: Huang, Yanyan1; Zhao, Rui1; Fu, Yabin2; Zhang, Qundan1; Xiong, Shaoxiang1; Li, Li2; Zhou, Rouli2; Liu, Guoquan1; Chen, Yi1
关键词: affinity chromatography ; cancer ; cell imaging ; molecular recognition ; peptides
刊名: CHEMBIOCHEM
发表日期: 2011-05-16
DOI: 10.1002/cbic.201100031
卷: 12, 期:8, 页:1209-1215
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: PERFORMANCE AFFINITY-CHROMATOGRAPHY ; ANTISENSE PEPTIDE ; SMALL MOLECULES ; HEPATOCELLULAR-CARCINOMA ; RECOGNITION ; TUMORS ; INHIBITORS ; LIBRARIES ; PROTEINS ; DELIVERY
英文摘要:

Specific detection and in vivo tracing of cancer biomarkers are important for cancer analysis. In this work, a simple and effective strategy for developing peptide probes was established. Peptides were rationally designed by using an antisense peptide approach directed towards an extracellular fragment (EL2) of a novel tumor-related protein LAPTM4B. Positional-scanning and stepwise affinity screening was employed to obtain an optimal peptide AP2H (IHGHHIISVG). The dissociation constant between the two small peptides, AP2H and the target EL2, was 5.51 mu m under physiological conditions. Fluorescence imaging assays indicated that AP2H can recognize live hepatoma cells by targeting the LAPTM4B protein on the cell surface with high specificity, low cytotoxicity and desirable cell penetrability. Compared to negative control cells, AP2H could differentiate cells with different expression levels of LAPTM4B. The screened peptide probe for molecular signatures of cancer cells, based on targeting the LAPTM4B protein, has potential applications in cancer diagnosis and targetable drug delivery.

语种: 英语
所属项目编号: 20975102 ; 21075125 ; 2007CB714504 ; CMS-CX200912
项目资助者: National Natural Science Foundation of China ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Institute of Chemistry, CAS
WOS记录号: WOS:000290862400011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/58952
Appears in Collections:基础医学院_细胞生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Peking Univ, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Yanyan,Zhao, Rui,Fu, Yabin,et al. Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides[J]. CHEMBIOCHEM,2011,12(8):1209-1215.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Yanyan]'s Articles
[Zhao, Rui]'s Articles
[Fu, Yabin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Yanyan]‘s Articles
[Zhao, Rui]‘s Articles
[Fu, Yabin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace