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Basic fibroblast growth factor promotes the development of human ovarian early follicles during growth in vitro
Wang, Tian-ren1,2; Yan, Li-ying1,3; Yan, Jie1,4; Lu, Cui-ling1,3; Xia, Xi1,7; Yin, Tai-lang1,3; Zhu, Xiao-hui1,4; Gao, Jiang-man1,4; Ding, Ting1,3; Hu, Wei-hong6; Guo, Hong-yan5; Li, Rong1,4; Qiao, Jie1,3,4
关键词human follicle in vitro 3D culture basic fibroblast growth factor in vitro growth
刊名HUMAN REPRODUCTION
2014-03-01
DOI10.1093/humrep/det465
29期:3页:568-576
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]LONG-TERM CULTURE ; ENCAPSULATED 3-DIMENSIONAL CULTURE ; PRIMORDIAL FOLLICLES ; PREANTRAL FOLLICLES ; STIMULATING-HORMONE ; MOUSE OOCYTES ; FOLLICULAR DEVELOPMENT ; SUPPORTS DEVELOPMENT ; KIT-LIGAND ; FACTOR FGF
英文摘要

STUDY QUESTION: What is the effect of basic fibroblast growth factor (bFGF) on the growth of individual early human follicles in a three-dimensional (3D) culture system in vitro?

SUMMARY ANSWER: The addition of 200 ng bFGF/ml improves human early follicle growth, survival and viability during growth in vitro.

WHAT IS KNOWN ALREADY: It has been demonstrated that bFGF enhances primordial follicle development in human ovarian tissue culture. However, the growth and survival of individual early follicles in encapsulated 3D culture have not been reported.

STUDY DESIGN, SIZE, DURATION: The maturation in vitro of human ovarian follicles was investigated. Ovarian tissue (n = 11) was obtained from 11 women during laparoscopic surgery for gynecological disease, after obtaining written informed consent. One hundred and fifty-four early follicles were isolated by enzymic digestion and mechanical disruption. They were individually encapsulated into alginate (1% w/v) and randomly assigned to be cultured with 0, 100, 200 or 300 ng bFGF/ml for 8 days.

PARTICIPANTS/MATERIALS, SETTING, METHODS: Individual follicles were cultured in minimum essential medium alpha (alpha MEM) supplemented with bFGF. Follicle survival and growth were assessed by microscopy. Follicle viability was evaluated under confocal laser scanning microscope following Calcein-AM and Ethidium homodimer-I (Ca-AM/EthD-I) staining.

MAIN RESULTS AND THE ROLE OF CHANCE: After 8 days in culture, all 154 follicles had increased in size. The diameter and survival rate of the follicles and the percentage with good viability were significantly higher in the group cultured with 200 ng bFGF/ml than in the group without bFGF (P < 0.05). The percentage of follicles in the pre-antral stage was significantly higher in the 200 ng bFGF/ml group than in the group without bFGF (P < 0.05), while the percentages of primordial and primary follicles were significantly lower (P < 0.05).

LIMITATIONS, REASONS FOR CAUTION: The study focuses on the effect of bFGF on the development of individual human early follicles in 3D culture in vitro and has limited ability to reveal the specific effect of bFGF at each different stage. The findings highlight the need to improve the acquisition and isolation of human ovarian follicles.

WIDER IMPLICATIONS OF THE FINDINGS: The in vitro 3D culture of human follicles with appropriate dosage of bFGF offers an effective method to investigate their development. Moreover, it allows early follicles to be cultured to an advanced stage and therefore has the potential to become an important source of mature oocytes for assisted reproductive technology; particularly as an option for fertility preservation in women, including patients with cancer.

语种英语
WOS记录号WOS:000331822100023
项目编号2011\CB944504 ; 2011CB944503 ; 81200470 ; 81000275 ; 31230047 ; 81101971
资助机构National Basic Research Program of China ; National Natural Science Foundation of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58956
专题北京大学第三临床医学院_生殖医学中心
北京大学第三临床医学院_妇产科
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Ctr Reprod Med, Beijing 100191, Peoples R China
3.China Med Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Shengjing Hosp, Shenyang 100004, Peoples R China
4.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
5.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
6.Gen Hosp Armed Police Forces, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
7.Guangdong Med Coll, Affiliated Shenzhen Nanshan Hosp, Dept Gynecol & Obstet, Shenzhen 518052, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Wang, Tian-ren,Yan, Li-ying,Yan, Jie,et al. Basic fibroblast growth factor promotes the development of human ovarian early follicles during growth in vitro[J]. HUMAN REPRODUCTION,2014,29(3):568-576.
APA Wang, Tian-ren.,Yan, Li-ying.,Yan, Jie.,Lu, Cui-ling.,Xia, Xi.,...&Qiao, Jie.(2014).Basic fibroblast growth factor promotes the development of human ovarian early follicles during growth in vitro.HUMAN REPRODUCTION,29(3),568-576.
MLA Wang, Tian-ren,et al."Basic fibroblast growth factor promotes the development of human ovarian early follicles during growth in vitro".HUMAN REPRODUCTION 29.3(2014):568-576.
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