|Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis|
|Yang, Xin1; Zhu, Tian-Yue1; Wen, Li-Cheng1; Cao, Yong-Ping1; Liu, Chao2; Cui, Yun-Peng1; Meng, Zhi-Chao1; Liu, Heng1|
|关键词||Intraarticular Injection Mesenchymal Stem Cell Osteoarthritis|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||HUMAN ARTICULAR-CARTILAGE ; STROMAL CELLS ; RAT MODEL ; FEMORAL-HEAD ; BONE ; DEFECTS ; REPAIR ; THERAPY ; TRANSPLANTATION ; IMMOBILIZATION|
Background: Researchers initially proposed the substitution of apoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells (MSCs) intraarticularly. This effect was termed as bio-resurfacing. Little evidence supporting the treatment of osteoarthritis (OA) by the delivery of a MSC suspension exists. The aim of this study was to investigate the effects of injecting allogenic MSCs intraarticularly in a rat OA model and to evaluate the influence of immobility on the effects of this treatment.
Methods: We established a rat knee OA model after 4 and 6 weeks and cultured primary bone marrow MSCs. A MSC suspension was injected into the articular space once per week for 3 weeks. A subgroup of knee joints was immobilized for 3 days after each injection, while the remaining joints were nonimmobilized. We used toluidine blue staining, Mankin scores, and TdT-mediated dUTP-biotin nick end labeling staining to evaluate the therapeutic effect of the injections. Comparisons between the therapy side and the control side of the knee joint were made using paired t-test, and comparisons between the immobilized and nonimmobilized subgroups were made using the unpaired t-test. A P value < 0.05 was considered significant.
Results: The three investigative approaches revealed less degeneration on the therapy sides of the knee joints than the control sides in both the 4- and 6-week groups (P < 0.05), regardless of immobilization. No significant differences were observed between the immobilized and nonimmobilized subgroups (P > 0.05).
Conclusions: Therapy involving the intraarticular injection of allogenic MSCs promoted cartilage repair in a rat arthritis model, and 3-day immobility after injection had little effect on this therapy.
|作者单位||1.Peking Univ, Dept Orthoped, Hosp 1, Beijing 100034, Peoples R China|
2.Third Mil Med Univ, Dept Orthoped, Xin Qiao Hosp, Chongqing 400037, Peoples R China
|Yang, Xin,Zhu, Tian-Yue,Wen, Li-Cheng,et al. Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis[J]. CHINESE MEDICAL JOURNAL,2015,128(18):2516-2523.|
|APA||Yang, Xin.,Zhu, Tian-Yue.,Wen, Li-Cheng.,Cao, Yong-Ping.,Liu, Chao.,...&Liu, Heng.(2015).Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis.CHINESE MEDICAL JOURNAL,128(18),2516-2523.|
|MLA||Yang, Xin,et al."Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis".CHINESE MEDICAL JOURNAL 128.18(2015):2516-2523.|