IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes
Tian Ning1; Zhang Lu2; Lu DanYu2; Zheng JingGao1; Ma WanYun1; Li Ying2
关键词aging DNA methyltransferase chromosome oocyte in vivo
刊名CHINESE SCIENCE BULLETIN
2013-09-01
DOI10.1007/s11434-013-5935-7
58期:26页:3248-3255
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Program Research Fund of the Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program ; National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Program Research Fund of the Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]QUANTUM DOTS ; GENE-EXPRESSION ; METHYLATION ; AGE ; SENESCENCE ; ANEUPLOIDY ; MICE ; DEACETYLATION ; MICROSCOPY ; EXCITATION
英文摘要

This paper investigated the age-related changes in the expression patterns of maintenance methyltransferase (DNMT1) and de novo methyltransferases (DNMT3a, 3b, 3L) and the chromosome architecture in in-vivo matured mouse oocytes using two-photon laser-scanning microscope. Our results showed that (1) DNMT1 and DNMT3a, 3b, 3L in the oocytes of pubertal mice were located in the cortical region of oocyte cytoplasm. In aging groups, DNMT1 was also located in the cortical region. However, DNMT3a, 3b, 3L had a relatively wider distribution in the oocyte cytoplasm and appeared near the chromosomes. These differences between pubertal and aging groups suggested that aging might affect DNA methylation; (2) the expression of DNMT1, and DNMT3a, 3b in aging groups increased significantly compared to pubertal groups, while, the expression of DNMT3L decreased. These results might be explained by the compensation mechanism among DNMTs, which might be impervious to aging; (3) aging caused increased errors in the distribution and three-dimensional morphology of chromosomes, including the increased total volume and surface area, the high ratio of height to diameter of a circular cylinder enclosing the chromosomes (H/D). Our work provided morphological information for the studies of age-related decline in oocyte qualities.

语种英语
所属项目编号90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
资助者National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Program Research Fund of the Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program ; National Natural Science Foundation of China ; National Basic Research Program of China ; Doctoral Program Research Fund of the Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
WOS记录号WOS:000323741500013
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/58974
Collection北京大学基础医学院
作者单位1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Histol & Embryol, Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Tian Ning,Zhang Lu,Lu DanYu,et al. Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes[J]. CHINESE SCIENCE BULLETIN,2013,58(26):3248-3255.
APA Tian Ning,Zhang Lu,Lu DanYu,Zheng JingGao,Ma WanYun,&Li Ying.(2013).Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes.CHINESE SCIENCE BULLETIN,58(26),3248-3255.
MLA Tian Ning,et al."Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes".CHINESE SCIENCE BULLETIN 58.26(2013):3248-3255.
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